Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line.

Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line. Research Abstract Details 

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Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line. Abstract Text:

Yoshitomo Honda,Takahisa Anada,Shinji Kamakura,Masanori Nakamura,Shunji Sugawara,Osamu Suzuki,

It has been suggested that macrophages and multinucleated giant cells are responsible for phagocytosis of resorbable calcium phosphate (CaP) compounds implanted in bone defects. However, function of macrophages around the CaP, if continuously exposed to various concentration of extracellular calcium ions ([Ca(2+)](o)), is still unknown. The present study showed that when resorbable octacalcium phosphate was implanted in mouse calvaria, macrophage-like cells were observed around the implant during bone formation. Then, experiments were designed to investigate whether secretion of bone morphogenetic protein 2 (BMP-2) is enhanced by [Ca(2+)](o) in a macrophage cell line (J774A.1) in vitro. The mRNA expression and the secretion of BMP-2 in J774A.1 cells were significantly increased when incubated in the medium with [Ca(2+)](o) up to 14mM. The promotion of mRNA expression was maintained even when incubated with a small amount of minute CaP crystals. The present results suggest that [Ca(2+)](o) above physiological concentration may stimulate macrophages to induce osteogenic cytokine, such as BMP-2, for bone formation by osteoblast.

Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line. Publishing Authors By Initials

Y Honda,T Anada,S Kamakura,M Nakamura,S Sugawara,O Suzuki,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 345

Page Numbers: 1155-60

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 0006-291X

DAY: 11

MONTH: 05

YEAR: 2006

Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line. Information

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LANGUAGE: eng

NlmUniqueID: 372516

Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line.

AFFILIATION: Division of Craniofacial Function Engineering (CFE), Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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