Elevated expression of genes under the control of stress response element (STRE) and Msn2p in an ethanol-tolerance sake yeast Kyokai no. 11.

Elevated expression of genes under the control of stress response element (STRE) and Msn2p in an ethanol-tolerance sake yeast Kyokai no. 11. Research Abstract Details 

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Elevated expression of genes under the control of stress response element (STRE) and Msn2p in an ethanol-tolerance sake yeast Kyokai no. 11. Abstract Text:

Mamoru Watanabe,Kenichi Tamura,Jose Paolo Magbanua,Kaname Takano,Katsuhiko Kitamoto,Hiroshi Kitagaki,Takeshi Akao,Hitoshi Shimoi,Mamoru Watanabe,Kenichi Tamura,Jose Paolo Magbanua,Kaname Takano,Katsuhiko Kitamoto,Hiroshi Kitagaki,Takeshi Akao,Hitoshi Shimoi,

The sake yeast strain Kyokai no. 11 (K11) is an ethanol-tolerant mutant of strain Kyokai no. 7 (K7), which shows higher viability in an ethanol solution than strain K7. To clarify the mechanism underlying the ethanol tolerance of this strain, the gene expression profiles of K7 and K11 were analyzed using DNA microarrays. The results indicate that many genes induced by stresses were highly expressed in strain K11 not exposed to stresses. Analysis of HSP12, one of the most highly expressed genes in strain K11 compared with strain K7, revealed that a trans-acting factor of strain K11 was involved in the elevated expression of HSP12. Many of the highly expressed genes in strain K11 including HSP12 were under the control of a cis-acting factor called the stress response element (STRE). The addition of STRE sequences to a promoter region of a reporter gene resulted in constitutive high-level expression in strain K11. It was reported that transcription factors Msn2p and Msn4p bind to STRE sequences. DNA sequence analyses of MSN2 and MSN4 of strains K7 and K11 revealed that only Msn2p was functional in these strains. When two copies of MSN2 in strain K11 were disrupted, the expression level of the reporter gene under the control of STRE decreased to the level of strain K7, indicating that Msn2p is required for the elevated expression of the STRE-controlled genes in K11.

Elevated expression of genes under the control of stress response element (STRE) and Msn2p in an ethanol-tolerance sake yeast Kyokai no. 11. Publishing Authors By Initials

M Watanabe,K Tamura,JP Magbanua,K Takano,K Kitamoto,H Kitagaki,T Akao,H Shimoi,M Watanabe,K Tamura,JP Magbanua,K Takano,K Kitamoto,H Kitagaki,T Akao,H Shimoi,

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Elevated expression of genes under the control of stress response element (STRE) and Msn2p in an ethanol-tolerance sake yeast Kyokai no. 11. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of bioscience and bioengineering

VOLUME: 104

Page Numbers: 163-70

Journal Abbreviation: J. Biosci. Bioeng.

ISSN: 1389-1723

DAY: 29

MONTH: Sep

YEAR: 2007

Elevated expression of genes under the control of stress response element (STRE) and Msn2p in an ethanol-tolerance sake yeast Kyokai no. 11. Information

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LANGUAGE: eng

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AFFILIATION: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashihiroshima 739-8530, Japan.

Country: Japan

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MEDLINETA: J Biosci Bioeng

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