Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from Arabidopsis leaves.

Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from Arabidopsis leaves. Research Abstract Details 

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Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from Arabidopsis leaves. Abstract Text:

Fong-Fu Hsu,John Turk,Todd D Williams,Ruth Welti,

Phosphatidylglycerol (PG) is the major phospholipid of plant chloroplasts. PG from Arabidopsis thaliana has an unusual fatty acyl chain, 3-trans-hexadecenoyl (Delta(3)16:1) in the sn-2 position of the major 18:3/Delta(3)16:1-PG species, as well as in 18:2/Delta(3)16:1-PG and 16:0/Delta(3)16:1-PG. Upon low-energy collisionally activated dissociation (CAD) in a tandem quadrupole or in an ion-trap mass spectrometer, the [M - H]- ions of the PG molecules containing Delta(3)16:1 give product-ion spectra that are readily distinguishable from those arising from PGs without the Delta(3)16:1 species. The Delta(3)16:1-fatty acyl-containing PGs are characterized by MS(2) product-ion mass spectra that contain predominant [M - H - 236]- ions arising from loss of the Delta(3)16:1-fatty acyl substituent as a ketene. This is attributable to the fact that the alpha-hydrogen of the Delta(3)16:1-fatty acid substituent involved in the ketene loss is an allylic hydrogen, which is very labile. This leads to preferential neutral loss of 236 and drastic decline in the neutral loss of 254 (i.e., loss as a fatty acid), the unique features that signify the presence of Delta(3)16:1-fatty acyl containing PGs. The neutral loss scan of 236, thus, provides a sensitive tandem quadrupole mass spectrometric means to identify Delta(3)16:1-containing PG species in lipid mixtures. This low-energy tandem mass spectrometric approach also permits the structures of the Arabidopsis PGs that consist of two isomeric structures to be unveiled.

Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from Arabidopsis leaves. Publishing Authors By Initials

FF Hsu,J Turk,TD Williams,R Welti,

For similar investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research abstracts see: investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research

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Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from Arabidopsis leaves. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Society for Mass Spectrome

VOLUME: 18

Page Numbers: 783-90

Journal Abbreviation: J. Am. Soc. Mass Spectrom.

ISSN: 1044-0305

DAY: 14

MONTH: 02

YEAR: 2007

Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from Arabidopsis leaves. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9010412

Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from Arabidopsis leaves. Keywords Mesh Terms:

KEYWORDS: Tandem Mass Spectrometry

MESH TERMS: methods

Chemical & Substance for Abstract: Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from Arabidopsis leaves. Information

Substance Name: Phosphatidylglycerols

Registry Number: 0

Grant and Affiliation Information for Electrospray ionization multiple stage quadrupole ion-trap and tandem quadrupole mass spectrometric studies on phosphatidylglycerol from Arabidopsis leaves.

AFFILIATION: Mass Spectrometry Resource, Division of Endocrinology, Diabetes, Metabolism, and Lipid research, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri, USA. fhsu@im.wustl.edu

Country: United States

AGENCY: United States NIDDK

GRANT: R37-DK-34388

ACRONYM: DK

MEDLINETA: J Am Soc Mass Spectrom

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