Electroporation of the vasculature and the lung.

Electroporation of the vasculature and the lung. Research Abstract Details 

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Electroporation of the vasculature and the lung. Abstract Text:

Electroporation has proven to be a highly effective technique for the in vivo delivery of genes to a number of solid tissues. In most of the reported methods, DNA is injected into the target tissue and electrodes are placed directly on or in the tissue for application of the electric field. While this works well for solid tissues, there are many tissues and organs that are not amenable to such an approach. In this review I will focus on the development of electroporation protocols for two such tissues: the vasculature and the lung. Several methods for in vivo electroporation of the vasculature have been developed in recent years that deliver DNA to vessel segments from either the inside or outside of the vessel. The advantages and disadvantages of each are discussed, as are the applications for which they have been used. In more recent work, our laboratory has developed a novel method to deliver genes to the rodent lung that results in high level, uniform, gene expression throughout all cell types of the lung. Most importantly, this technique is safe, and causes no inflammatory response or alterations in normal physiology of the organs. Taken together, these studies demonstrate the utility of electroporation for gene transfer to non injectible tissues.

Electroporation of the vasculature and the lung. Publishing Authors By Initials

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

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Electroporation of the vasculature and the lung. Journal Published:

PUBLICATION TYPE: Review

Journal: DNA and cell biology

VOLUME: 22

Page Numbers: 797-806

Journal Abbreviation: DNA Cell Biol.

ISSN: 1044-5498

DAY: 28

MONTH: Dec

YEAR: 2003

Electroporation of the vasculature and the lung. Information

Number of References: 49

LANGUAGE: eng

NlmUniqueID: 9004522

Electroporation of the vasculature and the lung. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: genetics

Chemical & Substance for Abstract: Electroporation of the vasculature and the lung. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Electroporation of the vasculature and the lung.

AFFILIATION: Division of Pulmonary and Critical Care Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA. dean@northwestern.edu

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL059956-06A2

ACRONYM: HL

MEDLINETA: DNA Cell Biol

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