Electrophysical monitoring of culture process of recombinant Escherichia coli strains.

Electrophysical monitoring of culture process of recombinant Escherichia coli strains. Research Abstract Details 

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Electrophysical monitoring of culture process of recombinant Escherichia coli strains. Abstract Text:

A novel method for monitoring the cell culture process has been developed. The method is based on the measurements of electro-optical characteristics of cell suspension, calculation of cell structure parameters, and the relationship between accumulation of proteins and change of these parameters' employment. Application of the method for the monitoring of a culture process of a recombinant strain is considered. The process of growth of recombinant strains cannot be sufficiently predicted and the direct measurement of cell culture parameters is unlikely to be the most efficient way of solving the problem.Escherichia coli plasmid-free and recombinant strains synthesizing the fusion protein consisting of tumor necrosis factor-alpha (TNF) and thymosin-alpha(1) (T) were studied. It was found that cytoplasmic electroconductivity of the strains investigated increased during the culture process. The accumulation of insoluble recombinant pThy-315-encoded hybrid protein TNF(SINGLEBOND)T in cells resulted in a decrease of the membrane dielectric permeability. To determine variations of membrane dielectric permeability the amount of insoluble recombinant protein TNF(SINGLEBOND)T in the bacterial cells should be calculated.

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Electrophysical monitoring of culture process of recombinant Escherichia coli strains. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology and bioengineering

VOLUME: 51

Page Numbers: 720-4

Journal Abbreviation: Biotechnol. Bioeng.

ISSN: 0006-3592

DAY: 20

MONTH: Sep

YEAR: 1996

Electrophysical monitoring of culture process of recombinant Escherichia coli strains. Information

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LANGUAGE: eng

NlmUniqueID: 7502021

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Grant and Affiliation Information for Electrophysical monitoring of culture process of recombinant Escherichia coli strains.

AFFILIATION: State Research Institute of Applied Microbiology, Obolensk, Moscow Reg., Russia 142279.

Country: United States

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MEDLINETA: Biotechnol Bioeng

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