Electron transfer reactions of chemically modified plastocyanin with P700 and cytochrome f. Importance of local charges.

Electron transfer reactions of chemically modified plastocyanin with P700 and cytochrome f. Importance of local charges. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Electron transfer reactions of chemically modified plastocyanin with P700 and cytochrome f. Importance of local charges. Abstract Text:

T Takabe,H Ishikawa,S Niwa,Y Tanaka,

Chemically modified spinach plastocyanin, in which negatively charged carboxyl residues are replaced with positively charged amino residues, has been prepared. Four distinct species of chemically modified plastocyanin, having 1 to 4 mol of modified carboxyl residue per mol of plastocyanin, could be separated by ion-exchange chromatography on DEAE-Sephacel. The rate of electron transfer from reduced cytochrome f to oxidized singly substituted plastocyanin was 30% of that of the native unmodified plastocyanin, and the reaction rate decreased further with increasing number of modified carboxyl residues. These results indicate the importance of electrostatic interactions between the negative charges on plastocyanin and the positive charges on cytochrome f in this reaction. Since the overall net charge of cytochrome f is negative at neutral pH, the positive charges on cytochrome f involved in the reaction should be localized ones. On the other hand, the rates of electron transfer from reduced singly and doubly substituted plastocyanin to photooxidized P700 in the P700-chlorophyll alpha protein complex were similar to that of native plastocyanin, which suggests that these carboxyl residues have only a minor role in the electron transfer to P700. Although divalent cation is essential for the electron transfer from native plastocyanin to P700 at neutral pH, the triply substituted plastocyanin could donate electrons to P700 even without MgCl2, and the rate of this reaction reached the maximum at a low concentration of MgCl2 (less than 2.5 mM). The modification of four carboxyl residues per plastocyanin molecule activated this reaction to the maximum level without MgCl2.(ABSTRACT TRUNCATED AT 250 WORDS)

Electron transfer reactions of chemically modified plastocyanin with P700 and cytochrome f. Importance of local charges. Publishing Authors By Initials

T Takabe,H Ishikawa,S Niwa,Y Tanaka,

For similar proteins: metalloproteins: plastocyanin research abstracts see: proteins: metalloproteins: plastocyanin research

PUBMED ID PMID:

MEDLINE DATE:

Electron transfer reactions of chemically modified plastocyanin with P700 and cytochrome f. Importance of local charges. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 96

Page Numbers: 385-93

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1984

Electron transfer reactions of chemically modified plastocyanin with P700 and cytochrome f. Importance of local charges. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Electron transfer reactions of chemically modified plastocyanin with P700 and cytochrome f. Importance of local charges. Keywords Mesh Terms:

KEYWORDS: Plastocyanin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Electron transfer reactions of chemically modified plastocyanin with P700 and cytochrome f. Importance of local charges. Information

Substance Name: Cytochromes f

Registry Number: 9035-46-5

Grant and Affiliation Information for Electron transfer reactions of chemically modified plastocyanin with P700 and cytochrome f. Importance of local charges.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Electron transfer reactions of chemically modified plastocyanin with P700 and cytochrome f Importance of local charges Related Publications

• A clonal analysis of neural progenitors during axolotl spinal cord regeneration reveals evidence for both spatially restricted and multipotent progenitors.
• Ankle skeletal injury predictions using anisotropic inelastic constitutive model of cortical bone taking into account damage evolution.
• Antiviral substances from the root of paeonia species.
• Application of a fine thread beam to the structure analysis of a hemihedrally twinned crystal of hydroxylamine oxidoreductase.
• Application of in vitro alternative methods to ecotoxicology.
• Association of Activities of Daily Living and Indices of Mental Status with Subsequent 20-year All-Cause Mortality in an Elderly Japanese Population.
• Circadian and light-induced transcription of clock gene Per1 depends on histone acetylation and deacetylation.
• Concentration of copper and a copper-EDTA complex at the pH junction formed in soil by an electrokinetic remediation process.
• Degradation of Ginseng Saponins under Mild Acidic Conditions.
• Diuretic action of paeonol.
• Effects Of Habitual Exercise And Gene Polymorphism On Quantitative Ultrasound Parameters In Japanese Elderly: 1291: Board #54 May 30 11:00 AM -12:30 PM.
• Ferritization treatment of copper in soil by electrokinetic remediation.
• Focal hepatic lesions mimicking cavernous hemangioma supplied by the portal vein.
• Further Study on Dammarane-Saponins of Leaves and Stems of American Ginseng, Panax quinquefolium.
• Improvement of the resonant cavity applicator for brain tumor hyperthermia - Computer simulation results -.
• Improvement of the resonant cavity applicator for brain tumor hyperthermia - Experimental heating results -.
• Influence of nutrient deficiency caused by host developmental arrest on the growth and development of a koinobiont parasitoid.
• Inhibition of NF-kappa B transcriptional activity by alpha-tocopheryl succinate.
• Optimal replication of poliovirus within cells.
• Panax ginseng: Relation between Age of Plant and Content of Ginsenosides.
• Proposal of a high-field superconducting wiggler for a slow positron source at SPring-8.
• Purification and Characterization of Antiviral Substance from the Bud of Syzygium aromatica.
• Seasonal variation of saponins, sucurose and monosaccharides in cultivated ginseng roots.
• Solubilization of saponins of bupleuri radix with ginseng saponins: cooperative effect of dammarane saponins.
• Studies on the Tissue Culture of Stevia rebaudiana and Its Components; (II)1. Induction of Shoot Primordia.
• Temperature distributions of developed needle type applicator.
• Tissue plasminogen activator and thrombin generation measurements using the Thrombinoscope.
• [Characteristics of polycyclic aromatic hydrocarbons (PAHS) on airborne particulates in Beijing]
• [Comparing identification of 3 kinds of flos chrysanthemi on surface morphology]
• [Drought tolerance of transgenic sweet potato expressing both Cu/Zn superoxide dismutase and ascorbate peroxidase]