Electron transfer reactions between cytochrome f and plastocyanin from Brassica komatsuna.

Electron transfer reactions between cytochrome f and plastocyanin from Brassica komatsuna. Research Abstract Details 

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Electron transfer reactions between cytochrome f and plastocyanin from Brassica komatsuna. Abstract Text:

S Niwa,H Ishikawa,S Nikai,T Takabe,

The protein-protein electron transfer reactions between cytochrome f and plastocyanin, both purified from Brassica komatsuna (Brassica rapa L. var. pervirdis Bailey), have been studied as a function of pH, ionic strength, and temperature. The second-order rate constant for the oxidation of ferrocytochrome f by plastocyanin was found to be k = 4.5 X 10(7) M-1 x S-1 at pH 7.0 mu 0.2 M, and 20 degrees C, with activation parameters delta H not equal to = 8.4 kcal/mol and delta S not equal to = 4.9 cal/mol x deg. Respective rate constant and activation parameters obtained for the reduction of ferricytochrome f by plastocyanin were k = 1.9 X 10(7) M-1 x S-1, delta H not equal to = 8.6 kcal/mol, and delta S not equal to = 3.9 cal/mol x deg. The high rate constants for these reactions and delta S not equal to = 4.9 cal/mol x deg. Respective rate constant and activation parameters obtained for the reduction of ferricytochrome f by plastocyanin were k = 1.9 X 10(7) M-1 x S-1, delta H not equal to = 8.6 kcal/mol, and delta S not equal to = 3.9 cal/mol x deg. The high rate constants for these reactions are attributable not to a low activation enthalpy but to a positive activation entropy term. The rate constants both for the oxidation and the reduction of cytochrome f by plastocyanin drastically decreased with increasing ionic strength, indicating the importance of electrostatic interactions. Divalent cations are more effective than monovalent cations in reducing the rates of these reactions. The rate constants for the oxidation of cytochrome f by plastocyanin are constant between pH 6.0 and 9.0 but decrease markedly above pH 9.0 and below pH 6.0. In the case of the reduction of cytochrome f by plastocyanin, an optimum pH around 7.0 was obtained and a biphasic feature was observed at alkaline pH. The results are discussed in relation to photosynthetic electron transport systems.

Electron transfer reactions between cytochrome f and plastocyanin from Brassica komatsuna. Publishing Authors By Initials

S Niwa,H Ishikawa,S Nikai,T Takabe,

For similar proteins: metalloproteins: plastocyanin research abstracts see: proteins: metalloproteins: plastocyanin research

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Electron transfer reactions between cytochrome f and plastocyanin from Brassica komatsuna. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 88

Page Numbers: 1177-83

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1980

Electron transfer reactions between cytochrome f and plastocyanin from Brassica komatsuna. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Electron transfer reactions between cytochrome f and plastocyanin from Brassica komatsuna. Keywords Mesh Terms:

KEYWORDS: Plastocyanin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Electron transfer reactions between cytochrome f and plastocyanin from Brassica komatsuna. Information

Substance Name: Cytochromes f

Registry Number: 9035-46-5

Grant and Affiliation Information for Electron transfer reactions between cytochrome f and plastocyanin from Brassica komatsuna.

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Country: JAPAN

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MEDLINETA: J Biochem

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