Electron transfer dissociation of doubly sodiated glycerophosphocholine lipids.

Electron transfer dissociation of doubly sodiated glycerophosphocholine lipids. Research Abstract Details 

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Electron transfer dissociation of doubly sodiated glycerophosphocholine lipids. Abstract Text:

Xiaorong Liang,Jian Liu,Yves LeBlanc,Tom Covey,A Celeste Ptak,J Thomas Brenna,Scott A McLuckey,

The ability to generate gaseous doubly charged cations of glycerophosphocholine (GPC) lipids via electrospray ionization has made possible the evaluation of electron-transfer dissociation (ETD) for their structural characterization. Doubly sodiated GPC cations have been reacted with azobenzene radical anions in a linear ion trap mass spectrometer. The ion/ion reactions proceed through sodium transfer, electron-transfer, and complex formation. Electron-transfer reactions are shown to give rise to cleavage at each ester linkage with the subsequent loss of a neutral quaternary nitrogen moiety. Electron-transfer without dissociation produces [M + 2Na](+.) radical cations, which undergo collision-induced dissociation (CID) to give products that arise from bond cleavage of each fatty acid chain. The CID of the complex ions yields products similar to those produced directly from the electron-transfer reactions of doubly sodiated GPC, although with different relative abundances. These findings indicate that the analysis of GPC lipids by ETD in conjunction with CID can provide some structural information, such as the number of carbons, degree of unsaturation for each fatty acid substituent, and the positions of the fatty acid substituents; some information about the location of the double bonds may be present in low intensity CID product ions.

Electron transfer dissociation of doubly sodiated glycerophosphocholine lipids. Publishing Authors By Initials

X Liang,J Liu,Y LeBlanc,T Covey,AC Ptak,JT Brenna,SA McLuckey,

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Electron transfer dissociation of doubly sodiated glycerophosphocholine lipids. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Society for Mass Spectrome

VOLUME: 18

Page Numbers: 1783-8

Journal Abbreviation: J. Am. Soc. Mass Spectrom.

ISSN: 1044-0305

DAY: 26

MONTH: 07

YEAR: 2007

Electron transfer dissociation of doubly sodiated glycerophosphocholine lipids. Information

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LANGUAGE: eng

NlmUniqueID: 9010412

Electron transfer dissociation of doubly sodiated glycerophosphocholine lipids. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Mass, Electrospray Ionizat

MESH TERMS: methods

Chemical & Substance for Abstract: Electron transfer dissociation of doubly sodiated glycerophosphocholine lipids. Information

Substance Name: Sodium

Registry Number: 7440-23-5

Grant and Affiliation Information for Electron transfer dissociation of doubly sodiated glycerophosphocholine lipids.

AFFILIATION: Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-2084, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 45,372

ACRONYM: GM

MEDLINETA: J Am Soc Mass Spectrom

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