Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain.

Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain. Abstract Text:

Jun Liu,Shenping Wu,Mary C Reedy,Hanspeter Winkler,Carmen Lucaveche,Yifan Cheng,Michael K Reedy,Kenneth A Taylor,

Subfragment 2 (S2), the segment that links the two myosin heads to the thick filament backbone, may serve as a swing-out adapter allowing crossbridge access to actin, as the elastic component of crossbridges and as part of a phosphorylation-regulated on-off switch for crossbridges in smooth muscle. Low-salt expansion increases interfilament spacing (from 52 nm to 67 nm) of rigor insect flight muscle fibers and exposes a tethering segment of S2 in many crossbridges. Docking an actoS1 atomic model into EM tomograms of swollen rigor fibers identifies in situ for the first time the location, length and angle assignable to a segment of S2. Correspondence analysis of 1831 38.7 nm crossbridge repeats grouped self-similar forms from which class averages could be computed. The full range of the variability in angles and lengths of exposed S2 was displayed by using class averages for atomic fittings of acto-S1, while S2 was modeled by fitting a length of coiled-coil to unaveraged individual repeats. This hybrid modeling shows that the average length of S2 tethers along the thick filament (except near the tapered ends) is approximately 10 nm, or 16% of S2's total length, with an angular range encompassing 90 degrees axially and 120 degrees azimuthally. The large range of S2 angles indicates that some rigor bridges produce positive force that must be balanced by others producing drag force. The short tethering segment clarifies constraints on the function of S2 in accommodating variable myosin head access to actin. We suggest that the short length of S2 may also favor intermolecular head-head interactions in IFM relaxed thick filaments.

Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain. Publishing Authors By Initials

J Liu,S Wu,MC Reedy,H Winkler,C Lucaveche,Y Cheng,MK Reedy,KA Taylor,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of molecular biology

VOLUME: 362

Page Numbers: 844-60

Journal Abbreviation:

ISSN: 0022-2836

DAY: 3

MONTH: 08

YEAR: 2006

Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985088

Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain.

AFFILIATION: Institute of Molecular Biophysics, Florida State University, FL 32306-4380, USA.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain Related Publications

• 1H and 13C NMR assignments and X-ray structures for three monocyclic benzoannelated dilactam polyethers.
• Activity of the dietary antioxidant ergothioneine in a virus gene-based assay for inhibitors of HIV transcription.
• Analytical parasitology.
• Blood oxygen studies in the premature.
• Cavernous hemangioma.
• Chemokine bioactivity of RANTES in endometriotic and normal endometrial stromal cells and peritoneal fluid.
• Disability, psychosocial, and demographic characteristics of abused women with physical disabilities.
• Early induction of parturition and initiation of lactation in sheep.
• Effects of gravity and hydrostatic pressure on angular acceleration coding sensory neurones in the crab and dogfish.
• Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain.
• For new CPR guidelines, think 30.
• Functional studies of an HIV-1 encoded glutathione peroxidase.
• Gene selection, alternative splicing, and post-translational processing regulate neuroligin selectivity for beta-neurexins.
• Imagery and craving in alcohol dependent persons.
• In vivo MR angiographic quantification of axial and twisting deformations of the superficial femoral artery resulting from maximum hip and knee flexion.
• Is vector body size the key to reduced malaria transmission in the irrigated region of Niono, Mali?
• Lung cancer chemoprevention: a randomized, double-blind trial in Linxian, China.
• Management of polycystic liver disease.
• Mechanoregulation of monocyte chemoattractant protein-1 expression in rat vascular smooth muscle cells.
• Medical informatics in medical research - the Severe Malaria in African Children (SMAC) Network's experience.
• New diagnoses of HTLV infection in England and Wales: 2002-2004.
• Posterior leukencephalopathy.
• Practical aspects of decontamination of the unconventional transmissible agents that cause sporadic and variant Creutzfeldt-Jakob disease and other similar human diseases.
• Recent advances in the diagnosis of heart failure.
• Societal costs of underage drinking.
• Standardization in immunohistochemistry: the role of antigen retrieval in molecular morphology.
• Stress burden, drug dependence and the nativity paradox among U.S. Hispanics.
• Syringocephaly.
• The evolution of hyperactivity, impulsivity and cognitive diversity.
• [Prevalence of lymphoma subtypes in Shanxi according to latest WHO classification]