Electron tomography of nascent herpes simplex virus virions.

Electron tomography of nascent herpes simplex virus virions. Research Abstract Details 

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Electron tomography of nascent herpes simplex virus virions. Abstract Text:

Joel D Baines,Chyong-Ere Hsieh,Elizabeth Wills,Carmen Mannella,Michael Marko,

Cells infected with herpes simplex virus type 1 (HSV-1) were conventionally embedded or freeze substituted after high-pressure freezing and stained with uranyl acetate. Electron tomograms of capsids attached to or undergoing envelopment at the inner nuclear membrane (INM), capsids within cytoplasmic vesicles near the nuclear membrane, and extracellular virions revealed the following phenomena. (i) Nucleocapsids undergoing envelopment at the INM, or B capsids abutting the INM, were connected to thickened patches of the INM by fibers 8 to 19 nm in length and < or =5 nm in width. The fibers contacted both fivefold symmetrical vertices (pentons) and sixfold symmetrical faces (hexons) of the nucleocapsid, although relative to the respective frequencies of these subunits in the capsid, fibers engaged pentons more frequently than hexons. (ii) Fibers of similar dimensions bridged the virion envelope and surface of the nucleocapsid in perinuclear virions. (iii) The tegument of perinuclear virions was considerably less dense than that of extracellular virions; connecting fibers were observed in the former case but not in the latter. (iv) The prominent external spikes emanating from the envelope of extracellular virions were absent from perinuclear virions. (v) The virion envelope of perinuclear virions appeared denser and thicker than that of extracellular virions. (vi) Vesicles near, but apparently distinct from, the nuclear membrane in single sections were derived from extensions of the perinuclear space as seen in the electron tomograms. These observations suggest very different mechanisms of tegumentation and envelopment in extracellular compared with perinuclear virions and are consistent with application of the final tegument to unenveloped nucleocapsids in a compartment(s) distinct from the perinuclear space.

Electron tomography of nascent herpes simplex virus virions. Publishing Authors By Initials

JD Baines,CE Hsieh,E Wills,C Mannella,M Marko,

For similar viruses: virion research abstracts see: viruses: virion research

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Electron tomography of nascent herpes simplex virus virions. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virology

VOLUME: 81

Page Numbers: 2726-35

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 10

MONTH: 01

YEAR: 2007

Electron tomography of nascent herpes simplex virus virions. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

Electron tomography of nascent herpes simplex virus virions. Keywords Mesh Terms:

KEYWORDS: Virion

MESH TERMS: ultrastructure

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Grant and Affiliation Information for Electron tomography of nascent herpes simplex virus virions.

AFFILIATION: Department of Microbiology and Immunology, C5132 Veterinary Medical Center, New York State College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. jdb11@cornell.edu

Country: United States

AGENCY: United States NCRR

GRANT: RR 12129

ACRONYM: RR

MEDLINETA: J Virol

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