The rate of oxygenation and that of trapping linoleic acid free radicals in the lipoxygenase [EC 1.13.11.12] reaction were measured in the presence of linoleic acid, oxygen, and nitrosobenzene at various concentrations, with a Clark oxygen electrode and ESR spectroscopy. The results were interpreted under the assumption that the free radical of linoleic acid, an intermediate of the lipoxygenase reaction, reacts competitively with oxygen or nitrosobenzene. The oxidation of the iron in the active site of lipoxygenase caused by the spin label reagent, 2-(10-carboxydecyl)-2-hexyl-4,4-dimethyl-3-oxazolidinyloxyl, was also observed by ESR- and fluorescence-spectroscopy.
Electron spin resonance studies on the lipoxygenase reaction by spin trapping and spin labelling methods. Publishing Authors By Initials
Electron spin resonance studies on the lipoxygenase reaction by spin trapping and spin labelling methods. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Journal of biochemistry
VOLUME: 82
Page Numbers: 1559-65
Journal Abbreviation: J. Biochem.
ISSN: 0021-924X
DAY: 19
MONTH: Dec
YEAR: 1977
Electron spin resonance studies on the lipoxygenase reaction by spin trapping and spin labelling methods. Information
Number of References:
LANGUAGE: eng
NlmUniqueID: 376600
Electron spin resonance studies on the lipoxygenase reaction by spin trapping and spin labelling methods. Keywords Mesh Terms:
KEYWORDS: Spin Labels
MESH TERMS: enzymology
Chemical & Substance for Abstract: Electron spin resonance studies on the lipoxygenase reaction by spin trapping and spin labelling methods. Information
Substance Name: Lipoxygenase
Registry Number: EC 1.13.11.12
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Country: JAPAN
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MEDLINETA: J Biochem
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