Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands.

Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands. Abstract Text:

Marco Piludu,Sean A Rayment,Bing Liu,Gwynneth D Offner,Frank G Oppenheim,Robert F Troxler,Arthur R Hand,

The human salivary mucins MG1 and MG2 are well characterized biochemically and functionally. However, there is disagreement regarding their cellular and glandular sources. The aim of this study was to define the localization and distribution of these two mucins in human salivary glands using a postembedding immunogold labeling method. Normal salivary glands obtained at surgery were fixed in 3% paraformaldehyde-0.1% glutaraldehyde and embedded in Lowicryl K4M or LR Gold resin. Thin sections were labeled with rabbit antibodies to MG1 or to an N-terminal synthetic peptide of MG2, followed by gold-labeled goat anti-rabbit IgG. The granules of all mucous cells of the submandibular and sublingual glands were intensely reactive with anti-MG1. No reaction was detected in serous cells. With anti-MG2, the granules of both mucous and serous cells showed reactivity. The labeling was variable in both cell types, with mucous cells exhibiting a stronger reaction in some glands and serous cells in others. In serous granules, the electron-lucent regions were more reactive than the dense cores. Intercalated duct cells near the acini displayed both MG1 and MG2 reactivity in their apical granules. In addition, the basal and lateral membranes of intercalated duct cells were labeled with anti-MG2. These results confirm those of earlier studies on MG1 localization in mucous cells and suggest that MG2 is produced by both mucous and serous cells. They also indicate differences in protein expression patterns among salivary serous cells.

Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands. Publishing Authors By Initials

M Piludu,SA Rayment,B Liu,GD Offner,FG Oppenheim,RF Troxler,AR Hand,

For similar salivary glands: submandibular gland research abstracts see: salivary glands: submandibular gland research

PUBMED ID PMID:

MEDLINE DATE:

Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The journal of histochemistry and cytochemistry :

VOLUME: 51

Page Numbers: 69-79

Journal Abbreviation: J. Histochem. Cytochem.

ISSN: 0022-1554

DAY: 29

MONTH: Jan

YEAR: 2003

Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9815334

Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands. Keywords Mesh Terms:

KEYWORDS: Submandibular Gland

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands. Information

Substance Name: Salivary Proteins

Registry Number: 0

Grant and Affiliation Information for Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands.

AFFILIATION: Departimento di Citomorfologia, Universita Degli Studi di Cagliari, Cagliari, Italy.

Country: United States

AGENCY: United States NIDDK

GRANT: DK44619

ACRONYM: DK

MEDLINETA: J Histochem Cytochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands Related Publications

• A Moderate Intensity Exercise Program Shows No Effect on Leisure and Occupational Physical Activity Levels: 2463: Board #10 June 2 8:00 AM - 9:30 AM.
• A total merit selection index for Ontario organic dairy farmers.
• Adenine as a growth factor for etiolated peas and its relation to the thermal inactivation of growth.
• Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana.
• China blasts off on lunar exploration.
• Circumcision and venereal disease.
• Diabetic acidosis and coma.
• Diagnosis and treatment of Sarcoptes scabiei infestations.
• Dissociation and symptom dimensions of obsessive-compulsive disorder : A replication study.
• Effects of Aerobic and Resistance Exercise TVaining on Strength in HIV-infected Men: 2394: Board #73 June 1 3:30 PM -5:00 PM.
• Effects of Combined Aerobic and Resistance Exercise TVaining in HIV-Infected Men: 2393: Board #72 June 1 3:30 PM -5:00 PM.
• Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands.
• Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase.
• Genetics of xanthoma tuberosum multiplex.
• Head in the clouds.
• Incidence of periodontal attachment loss over 8 to 10 years among Iowa elders aged 71+ at baseline.
• Informativeness of the spoken narratives of younger and older adolescents with specific language impairment and their counterparts with normal language.
• Interstitial cystitis; report of 223 cases (204 women and 19 men).
• Interstitial cystitis; report of 223 cases, 204 women and 19 men.
• Mass in buttocks.
• Moderate-Intensity Exercise Alters Circulating Hormones and Cytokines in HIV-Infected Men: 944: June 2 9:00 AM - 9:15 AM.
• Regulation of Iron Homeostasis Mediated by the Heme-binding Protein Dap1 (Damage Resistance Protein 1) via the P450 Protein Erg11/Cyp51.
• Revised algorithm for estimating light extinction from IMPROVE particle speciation data.
• Rupture of the spleen in an erythroblastotic infant.
• Salivary histatins in human deep posterior lingual glands (of von Ebner).
• Security issues plague US research.
• Space and astronomy: the girl next door.
• Studies on the physiology of light action; auxin and the light inhibition of growth.
• The effect of 3-indoleacetic acid on the response of Lactobacillus arabinosus 17-5 to nicotinamide.
• UK astronomers stunned by Gemini withdrawal.