Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells.

Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. Research Abstract Details 

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Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. Abstract Text:

Elizabeth R Wright,Jordan B Schooler,H Jane Ding,Collin Kieffer,Christopher Fillmore,Wesley I Sundquist,Grant J Jensen,

The major structural elements of retroviruses are contained in a single polyprotein, Gag, which in human immunodeficiency virus type 1 (HIV-1) comprises the MA, CA, spacer peptide 1 (SP1), NC, SP2, and p6 polypeptides. In the immature HIV-1 virion, the domains of Gag are arranged radially with the N-terminal MA domain at the membrane and C-terminal NC-SP2-p6 region nearest to the center. Here, we report the three-dimensional structures of individual immature HIV-1 virions, as obtained by electron cryotomography. The concentric shells of the Gag polyprotein are clearly visible, and radial projections of the different Gag layers reveal patches of hexagonal order within the CA and SP1 shells. Averaging well-ordered unit cells leads to a model in which each CA hexamer is stabilized by a bundle of six SP1 helices. This model suggests why the SP1 spacer is essential for assembly of the Gag lattice and how cleavage between SP1 and CA acts as a structural switch controlling maturation.

Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. Publishing Authors By Initials

ER Wright,JB Schooler,HJ Ding,C Kieffer,C Fillmore,WI Sundquist,GJ Jensen,

For similar viruses: virion research abstracts see: viruses: virion research

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Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The EMBO journal

VOLUME: 26

Page Numbers: 2218-26

Journal Abbreviation:

ISSN: 0261-4189

DAY: 29

MONTH: 03

YEAR: 2007

Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. Information

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LANGUAGE: eng

NlmUniqueID: 8208664

Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. Keywords Mesh Terms:

KEYWORDS: Virion

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. Information

Substance Name: Peptides

Registry Number: 0

Grant and Affiliation Information for Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells.

AFFILIATION: Division of Biology, California Institute of Technology, Pasadena, CA, USA.

Country: England

AGENCY: United States NIGMS

GRANT: P01 GM66521

ACRONYM: GM

MEDLINETA: EMBO J

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