Electrokinetic trapping and concentration enrichment of DNA in a microfluidic channel.

Electrokinetic trapping and concentration enrichment of DNA in a microfluidic channel. Research Abstract Details 

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Electrokinetic trapping and concentration enrichment of DNA in a microfluidic channel. Abstract Text:

Jinhua Dai,Takashi Ito,Li Sun,Richard M Crooks,

We report a simple and efficient method for enriching the concentration of charged analytes within microfluidic channels. The method relies on exerting spatial control over the electrokinetic velocity of an analyte. Specifically, the electroosmotic (eo) velocity of the buffer solution in one region of the microfluidic system opposes the electrophoretic (ep) velocity of the analyte in the other region. This results in ep transport of DNA to the location where the ep and eo velocities are equal and opposite. Accumulation of the analyte occurs at this location. This enrichment method is conceptually distinct from field-amplification stacking, isotachophoresis, micelle sweeping, size exclusion, and other methods that have been previously reported. The method requires no complex microfabricated structures, no special manipulation of the solvent, and the enriched analyte remains in solution rather than being captured on a solid support. A concentration enrichment factor of 800 can be achieved for 20mer DNA in a fluidic channel having dimensions of 100 mum x 25 mum x 5 mm. The time required to achieve this level of enrichment is 300 s, and the enriched zone has a minimum width of 100 mum.

Electrokinetic trapping and concentration enrichment of DNA in a microfluidic channel. Publishing Authors By Initials

J Dai,T Ito,L Sun,RM Crooks,

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Electrokinetic trapping and concentration enrichment of DNA in a microfluidic channel. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 13026-7

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 29

MONTH: Oct

YEAR: 2003

Electrokinetic trapping and concentration enrichment of DNA in a microfluidic channel. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Electrokinetic trapping and concentration enrichment of DNA in a microfluidic channel. Keywords Mesh Terms:

KEYWORDS: Silicones

MESH TERMS: chemistry

Chemical & Substance for Abstract: Electrokinetic trapping and concentration enrichment of DNA in a microfluidic channel. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Electrokinetic trapping and concentration enrichment of DNA in a microfluidic channel.

AFFILIATION: Department of Chemistry, Texas A&M University, P.O. Box 30012, College Station, TX 77842-3012, USA.

Country: United States

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MEDLINETA: J Am Chem Soc

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