Electrochemical surface plasmon resonance measurement in a microliter volume flow cell for evaluating the affinity and catalytic activity of biomolecules.

Electrochemical surface plasmon resonance measurement in a microliter volume flow cell for evaluating the affinity and catalytic activity of biomolecules. Research Abstract Details 

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Electrochemical surface plasmon resonance measurement in a microliter volume flow cell for evaluating the affinity and catalytic activity of biomolecules. Abstract Text:

Ryoji Kurita,Yoshimi Yokota,Akio Ueda,Osamu Niwa,Ryoji Kurita,Yoshimi Yokota,Akio Ueda,Osamu Niwa,Ryoji Kurita,Yoshimi Yokota,Akio Ueda,Osamu Niwa,

We developed an electrochemical surface plasmon resonance flow cell for the simultaneous measurement of the binding affinity and catalytic activity of bifunctional biomolecules. These measurements will be useful for evaluating the performance of such biomolecules as ribozyme and abzyme. The simultaneous measurements were performed on a gold surface modified with a multilayer consisting of poly-l-lysine and poly(styrene sulfonate) assembled with the layer-by-layer method using an enzyme-labeled monoclonal antibody as a model compound. We obtained the amount of immunocomplex formation from the surface plasmon resonance angle shift value by injecting the compound into the flow cell containing the multilayer modified with tumor necrosis factor-alpha. Then we compared this surface plasmon resonance result with that for the in situ electrochemical oxidation of p-aminophenol formed by the catalytic reaction of labeled enzyme on the same gold film. We were able to obtain a high correlation coefficient of 0.999 between the two responses. This is because the compound could be captured with high stability with a less than 3% coulometric response decrease in the catalyzed product in the multilayer whose thickness was easily controllable. In addition, we were able to measure the catalytic activity by coulometry and thus avoid the effect of peak broadening. We also report that the dephosphorylation activity of a bound compound could be estimated from the measurement results and an equation.

Electrochemical surface plasmon resonance measurement in a microliter volume flow cell for evaluating the affinity and catalytic activity of biomolecules. Publishing Authors By Initials

R Kurita,Y Yokota,A Ueda,O Niwa,R Kurita,Y Yokota,A Ueda,O Niwa,R Kurita,Y Yokota,A Ueda,O Niwa,

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Electrochemical surface plasmon resonance measurement in a microliter volume flow cell for evaluating the affinity and catalytic activity of biomolecules. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 9572-6

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 31

MONTH: 10

YEAR: 2007

Electrochemical surface plasmon resonance measurement in a microliter volume flow cell for evaluating the affinity and catalytic activity of biomolecules. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Electrochemical surface plasmon resonance measurement in a microliter volume flow cell for evaluating the affinity and catalytic activity of biomolecules.

AFFILIATION: National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, Japan, and Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Japan.

Country: United States

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MEDLINETA: Anal Chem

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