Electrochemical multianalyte immunoassays using an array-based sensor.

Electrochemical multianalyte immunoassays using an array-based sensor. Research Abstract Details 

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Electrochemical multianalyte immunoassays using an array-based sensor. Abstract Text:

Michael S Wilson,Weiyan Nie,

A novel amperometric biosensor for performing simultaneous electrochemical multianalyte immunoassays is described. The sensor consisted of eight iridium oxide sensing electrodes (0.78 mm(2) each), an iridium counter electrode, and a Ag/AgCl reference electrode patterned on a glass substrate. Four different capture antibodies were immobilized on the sensing electrodes via adsorption. Quantification of proteins was achieved using an ELISA in which the electrochemical oxidation of enzyme-generated hydroquinone was measured. The spatial separation of the electrodes enabled simultaneous electrochemical immunoassays for multiple proteins to be conducted in a single assay without amperometric cross-talk between the electrodes. The simultaneous detection of goat IgG, mouse IgG, human IgG, and chicken IgY was demonstrated. The detection limit was 3 ng/mL for all analytes. The sensor had excellent precision (1.9-8.2% interassay CV) and was comparable in performance to commercial single-analyte ELISAs. We anticipate that chip-based sensors, as described herein, will be suitable for the mass production of economical, miniaturized, multianalyte assay devices.

Electrochemical multianalyte immunoassays using an array-based sensor. Publishing Authors By Initials

MS Wilson,W Nie,

For similar inorganic chemicals: silver compounds research abstracts see: inorganic chemicals: silver compounds research

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Electrochemical multianalyte immunoassays using an array-based sensor. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical chemistry

VOLUME: 78

Page Numbers: 2507-13

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 15

MONTH: Apr

YEAR: 2006

Electrochemical multianalyte immunoassays using an array-based sensor. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Electrochemical multianalyte immunoassays using an array-based sensor. Keywords Mesh Terms:

KEYWORDS: Silver Compounds

MESH TERMS: chemistry

Chemical & Substance for Abstract: Electrochemical multianalyte immunoassays using an array-based sensor. Information

Substance Name: silver chloride

Registry Number: 7783-90-6

Grant and Affiliation Information for Electrochemical multianalyte immunoassays using an array-based sensor.

AFFILIATION: EIC Laboratories, Inc., 111 Downey Street, Norwood, Massachusetts 02062, USA. mwilson@eiclabs.com

Country: United States

AGENCY: United States NCI

GRANT: 2 R44 CA096250-02

ACRONYM: CA

MEDLINETA: Anal Chem

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