Electrochemical DNA biosensor using a disposable electrochemical printed (DEP) chip for the detection of SNPs from unpurified PCR amplicons.

Electrochemical DNA biosensor using a disposable electrochemical printed (DEP) chip for the detection of SNPs from unpurified PCR amplicons. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Electrochemical DNA biosensor using a disposable electrochemical printed (DEP) chip for the detection of SNPs from unpurified PCR amplicons. Abstract Text:

Minhaz Uddin Ahmed,Koutarou Idegami,Miyuki Chikae,Kagan Kerman,Piyasak Chaumpluk,Shohei Yamamura,Eiichi Tamiya,

In this study, we are reporting for the first time the elucidation of single nucleotide polymorphisms (SNPs) of clinically important alleles from consenting human subjects using a disposable electrochemical printed (DEP) chip in connection with differential pulse voltammetry (DPV) and a redox active molecule Hoechst 33258 [H33258, 2'-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi(1H-benzimidazole)]. Post-PCR products were analyzed directly without any purification process. The aggregation of the DNA-H33258 complex causes a significant drop in the peak current intensity of H33258 oxidation. The phenomenon of DNA aggregation induced by H33258 in addition to changes in anodic current peak are used to detect SNPs. Since laborious probe immobilization was not required, our biosensor offers several benefits due to its simplicity and rapid response as a promising device for genetic analysis.

Electrochemical DNA biosensor using a disposable electrochemical printed (DEP) chip for the detection of SNPs from unpurified PCR amplicons. Publishing Authors By Initials

MU Ahmed,K Idegami,M Chikae,K Kerman,P Chaumpluk,S Yamamura,E Tamiya,

For similar genetic phenomena: variation (genetics): polymorphism, genetic: polymorphism, single nucleotide research abstracts see: genetic phenomena: variation (genetics): polymorphism, genetic: polymorphism, single nucleotide research

PUBMED ID PMID:

MEDLINE DATE:

Electrochemical DNA biosensor using a disposable electrochemical printed (DEP) chip for the detection of SNPs from unpurified PCR amplicons. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Analyst

VOLUME: 132

Page Numbers: 431-8

Journal Abbreviation:

ISSN: 0003-2654

DAY: 14

MONTH: 03

YEAR: 2007

Electrochemical DNA biosensor using a disposable electrochemical printed (DEP) chip for the detection of SNPs from unpurified PCR amplicons. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372652

Electrochemical DNA biosensor using a disposable electrochemical printed (DEP) chip for the detection of SNPs from unpurified PCR amplicons. Keywords Mesh Terms:

KEYWORDS: Polymorphism, Single Nucleotide

MESH TERMS: methods

Chemical & Substance for Abstract: Electrochemical DNA biosensor using a disposable electrochemical printed (DEP) chip for the detection of SNPs from unpurified PCR amplicons. Information

Substance Name: Bisbenzimide

Registry Number: 23491-45-4

Grant and Affiliation Information for Electrochemical DNA biosensor using a disposable electrochemical printed (DEP) chip for the detection of SNPs from unpurified PCR amplicons.

AFFILIATION: School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi City, Ishikawa 923-1292, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Analyst

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Electrochemical DNA biosensor using a disposable electrochemical printed DEP chip for the detection of SNPs from unpurified PCR amplicons Related Publications

• A Rapid Sample Pretreatment Protocol: Improved Sensitivity in the Detection of a Low-abundant Serum Biomarker for Prostate Cancer.
• A method for micrometer resolution patterning of primary culture neurons for SPM analysis.
• A picoliter chamber array for cell-free protein synthesis.
• A rapid label-free electrochemical detection and kinetic study of Alzheimer's amyloid beta aggregation.
• Acid phosphatases from Fusarium moniliforme. Purification and enzymatic properties.
• DNA condensation monitoring after interaction with hoechst 33258 by atomic force microscopy and fluorescence spectroscopy.
• Detection of Alzheimer's tau protein using localised surface plasmon resonance-based immunochip.
• Electrochemical DNA biosensor using a disposable electrochemical printed (DEP) chip for the detection of SNPs from unpurified PCR amplicons.
• Electrochemical consideration on the optimum pH of bilirubin oxidase.
• Enzyme-linked sensitive fluorometric imaging of glutamate release from cerebral neurons of chick embryos.
• Fractionation of two diguanylic acids with different internucleotide linkages and its application for analyses of the synthetic products of ribonuclease T1 and spontaneous reaction.
• Gender issues in elderly care and possible solutions.
• INCORPORATION OF O18 FROM AIR INTO TYROSINE.
• Iodination of erabutoxin b: diiodohistidine formation.
• Label-free cell-based assay using localized surface plasmon resonance biosensor.
• Label-free optical detection of aptamer-protein interactions using gold-capped oxide nanostructures.
• Methionine sulfoxide in the resilium protein of surf clams.
• Microsystems technology and biosensing.
• Phospholipase A of sea snake Laticauda semifasciata venom. Isolation and properties of novel forms lacking tryptophan.
• Purification and characterization of cold-active L-glutamate dehydrogenase independent of NAD(P) and oxygen.
• Purification and properties of the acetylcholine receptor protein from Narke japonica.
• Refinement of a locus for autosomal dominant hereditary motor and sensory neuropathy with proximal dominancy (HMSN-P) and genetic heterogeneity.
• Role of algae as food.
• The acetylation of the amino groups of Laticauda semifasciata III, a sea snake venom component.
• Total syntheses of amphidinolide H and G.
• [A case report: mitral valve replacement for the patient with essential thrombocythemia]
• [Clinical features of ruptured chordae tendineae with special reference to mitral valve prolapse]
• [Doppler and echocardiographic study of normal systolic murmurs]
• [Experience with successful palliative surgical therapy for aneurysm of the descending aorta involving the aortic arch in a poor-risk aged patient]
• [Role of lysines in the stability of a snake neurotoxin]