Electrically actuated, pressure-driven liquid chromatography separations in microfabricated devices.

Electrically actuated, pressure-driven liquid chromatography separations in microfabricated devices. Research Abstract Details 

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Electrically actuated, pressure-driven liquid chromatography separations in microfabricated devices. Abstract Text:

Hernan V Fuentes,Adam T Woolley,Hernan V Fuentes,Adam T Woolley,

Electrolysis-based micropumps integrated with microfluidic channels in micromachined glass substrates are presented. Photolithography combined with wet chemical etching and thermal bonding enabled the fabrication of multi-layer devices containing electrically actuated micropumps interfaced with sample and mobile phase reservoirs. A stationary phase was deposited on the microchannel walls by coating with 10% (w/w) chlorodimethyloctadecylsilane in toluene. Pressure-balanced injection was implemented by controlling the electrolysis time and voltage applied in the two independent micropumps. Current fluctuations in the micropumps due to the stochastic formation of bubbles on the electrode surfaces were determined to be the main cause of variation between separations. On-chip electrochemical pumping enabled the loading of pL samples with no dead volume between injection and separation. A mobile phase composed of 70% acetonitrile and 30% 50 mM acetate buffer (pH 5.45) was used for the chromatographic separation of three fluorescently labeled amino acids in <40 s with an efficiency of >3000 theoretical plates in a 2.5 cm-long channel. Our results demonstrate the potential of electrochemical micropumps integrated with microchannels to perform rapid chromatographic separations in a microfabricated platform. Importantly, these devices represent a significant step toward the development of miniaturized and fully integrated liquid chromatography systems.

Electrically actuated, pressure-driven liquid chromatography separations in microfabricated devices. Publishing Authors By Initials

HV Fuentes,AT Woolley,HV Fuentes,AT Woolley,

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Electrically actuated, pressure-driven liquid chromatography separations in microfabricated devices. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Lab on a chip

VOLUME: 7

Page Numbers: 1524-31

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ISSN: 1473-0197

DAY: 10

MONTH: 08

YEAR: 2007

Electrically actuated, pressure-driven liquid chromatography separations in microfabricated devices. Information

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LANGUAGE: eng

NlmUniqueID: 101128948

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Grant and Affiliation Information for Electrically actuated, pressure-driven liquid chromatography separations in microfabricated devices.

AFFILIATION: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.

Country: England

AGENCY: United States NIBIB

GRANT: EB006124

ACRONYM: EB

MEDLINETA: Lab Chip

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