Elastin content correlates with human disc degeneration in the anulus fibrosus and nucleus pulposus.

Elastin content correlates with human disc degeneration in the anulus fibrosus and nucleus pulposus. Research Abstract Details 

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Elastin content correlates with human disc degeneration in the anulus fibrosus and nucleus pulposus. Abstract Text:

Jordan M Cloyd,Dawn M Elliott,

STUDY DESIGN: Quantitative study of elastin content in nondegenerate and degenerate human intervertebral discs. OBJECTIVE: To measure the site-specific changes in elastin content that accompany disc degeneration using a quantitative, dye-binding assay to assess elastin levels. SUMMARY OF BACKGROUND DATA: Recently, an abundant and organized network of elastic fibers was observed in nondegenerated human disc using immunostaining histochemistry, suggesting a functional role for elastin. While degenerative changes in the disc extracellular matrix composition are well known, changes in elastin content that may accompany degeneration have not been reported. METHODS: Human discs were assigned a degenerative grade by 3 independent orthopedic surgeons based on gross morphology. Samples were taken from the outer anulus fibrosus (OAF), inner AF (IAF) and nucleus pulposus (NP). Elastin content was measured using a specific, dye-binding assay and normalized to dry weight and collagen content, which was measured via a hydroxyproline assay. Samples were divided into 2 groups: nondegenerate (Grades 1-2.5) and degenerate (Grades 2.6-4.0). A 2-way analysis of variance was used to test for statistical significance where the 2 factors were disc location and degeneration. Correlations of composition with degeneration and age were analyzed. RESULTS: In nondegenerate tissue, elastin by dry weight was on average 2.0% +/- 0.3%, and there were no differences in elastin content among the locations of OAF, IAF, or NP. With degeneration, there was a significant increase in total disc elastin per dry weight at each location. The degenerate IAF had the largest amount of elastin (9.3% +/- 2.3%), significantly greater than the NP and OAF. Elastin content correlated with degenerative grade and age at each site. CONCLUSION: Based on the location-dependent degenerative changes, with highest increases in the IAF, elastin may function to restore lamellar structure under radial loads that potentially cause delamination. Future work will focus on distinguishing the changes in elastin orientation with degeneration and understanding the mechanical functional role of elastin in the disc.

Elastin content correlates with human disc degeneration in the anulus fibrosus and nucleus pulposus. Publishing Authors By Initials

JM Cloyd,DM Elliott,

For similar musculoskeletal diseases: bone diseases: spinal diseases research abstracts see: musculoskeletal diseases: bone diseases: spinal diseases research

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Elastin content correlates with human disc degeneration in the anulus fibrosus and nucleus pulposus. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Spine

VOLUME: 32

Page Numbers: 1826-31

Journal Abbreviation: Spine

ISSN: 1528-1159

DAY: 1

MONTH: Aug

YEAR: 2007

Elastin content correlates with human disc degeneration in the anulus fibrosus and nucleus pulposus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7610646

Elastin content correlates with human disc degeneration in the anulus fibrosus and nucleus pulposus. Keywords Mesh Terms:

KEYWORDS: Spinal Diseases

MESH TERMS: pathology

Chemical & Substance for Abstract: Elastin content correlates with human disc degeneration in the anulus fibrosus and nucleus pulposus. Information

Substance Name: Elastin

Registry Number: 9007-58-3

Grant and Affiliation Information for Elastin content correlates with human disc degeneration in the anulus fibrosus and nucleus pulposus.

AFFILIATION: McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA 19104-6081, USA.

Country: United States

AGENCY: United States NIBIB

GRANT: EB 002425

ACRONYM: EB

MEDLINETA: Spine

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