EKLF and KLF2 have compensatory roles in embryonic beta-globin gene expression and primitive erythropoiesis.

EKLF and KLF2 have compensatory roles in embryonic beta-globin gene expression and primitive erythropoiesis. Research Abstract Details 

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EKLF and KLF2 have compensatory roles in embryonic beta-globin gene expression and primitive erythropoiesis. Abstract Text:

Priyadarshi Basu,Tina K Lung,Wafaa Lemsaddek,Thanh Giang Sargent,David C Williams,Mohua Basu,Latasha C Redmond,Jerry B Lingrel,Jack L Haar,Joyce A Lloyd,Priyadarshi Basu,Tina K Lung,Wafaa Lemsaddek,Thanh Giang Sargent,David C Williams,Mohua Basu,Latasha C Redmond,Jerry B Lingrel,Jack L Haar,Joyce A Lloyd,

The Krüppel-like C2/H2 zinc finger transcription factors (KLFs) control development and differentiation. Erythroid Krüppel-like factor (EKLF or KLF1) regulates adult beta-globin gene expression and is necessary for normal definitive erythropoiesis. KLF2 is required for normal embryonic Ey- and betah1-, but not adult betaglobin, gene expression in mice. Both EKLF and KLF2 play roles in primitive erythroid cell development. To investigate potential interactions between these genes, EKLF/KLF2 double-mutant embryos were analyzed. EKLF(-/-)KLF2(-/-) mice appear anemic at embryonic day 10.5 (E10.5) and die before E11.5, whereas single-knockout EKLF(-/-) or KLF2(-/-) embryos are grossly normal at E10.5 and die later than EKLF(-/-)KLF2(-/-) embryos. At E10.5, Ey- and betah1-globin mRNA is greatly reduced in EKLF(-/-)KLF2(-/-), compared with EKLF(-/-) or KLF2(-/-) embryos, consistent with the observed anemia. Light and electron microscopic analyses of E9.5 EKLF(-/-)KLF2(-/-) yolk sacs, and cytospins, indicate that erythroid and endothelial cells are morphologically more abnormal than in either single knockout. EKLF(-/-)KLF2(-/-) erythroid cells are markedly irregularly shaped, suggesting membrane abnormalities. EKLF and KLF2 may have coordinate roles in a common progenitor to erythroid and endothelial cells. The data indicate that EKLF and KLF2 have redundant functions in embryonic beta-like globin gene expression, primitive erythropoiesis, and endothelial development.

EKLF and KLF2 have compensatory roles in embryonic beta-globin gene expression and primitive erythropoiesis. Publishing Authors By Initials

P Basu,TK Lung,W Lemsaddek,TG Sargent,DC Williams,M Basu,LC Redmond,JB Lingrel,JL Haar,JA Lloyd,P Basu,TK Lung,W Lemsaddek,TG Sargent,DC Williams,M Basu,LC Redmond,JB Lingrel,JL Haar,JA Lloyd,

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EKLF and KLF2 have compensatory roles in embryonic beta-globin gene expression and primitive erythropoiesis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Blood

VOLUME: 110

Page Numbers: 3417-25

Journal Abbreviation: Blood

ISSN: 0006-4971

DAY: 3

MONTH: 08

YEAR: 2007

EKLF and KLF2 have compensatory roles in embryonic beta-globin gene expression and primitive erythropoiesis. Information

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LANGUAGE: eng

NlmUniqueID: 7603509

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Grant and Affiliation Information for EKLF and KLF2 have compensatory roles in embryonic beta-globin gene expression and primitive erythropoiesis.

AFFILIATION: Department of Human Genetics, and Massey Cancer Center, Virginia Commonwealth University, 401 College Street, Richmond, VA 23298, USA.

Country: United States

AGENCY: United States NCI

GRANT: P30 CA 16059

ACRONYM: CA

MEDLINETA: Blood

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