Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography.

Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography. Abstract Text:

Kajal Chakraborty,R Paul Raj,

Eicosapentaenoic acid (EPA) derived from chemically hydrolyzed sardine oil was concentrated by urea fractionation using methanol at different temperatures (2, 4, and 6 degrees C) and urea/fatty acid ratios (2:1, 3:1, and 4:1 w/w) and purified by argentation neutral alumina column chromatography. The individual fatty acids were determined as fatty acid methyl esters (FAME) by gas-liquid chromatography and gas chromatography-mass spectroscopy as FAME and N-acyl pyrrolidides. In the mass fragmentation pattern of FAME, the base peak was assigned to be the 1-methoxyethenol moiety (m/z = 74) obtained by McLafferty rearrangement. Formation of the cyclic tropylium ion (m/z = 91) in fatty acids with four or more double bonds was apparent in FAME-PUFAs. The base peak of N-acyl pyrrolidides was the McLafferty rearrangement ion, 1-(pyrrolidin-1-yl)ethenol (m/z = 113). The highest concentration of EPA (47.78%) was obtained at the crystallization temperature of 4 degrees C with a urea/fatty acid ratio of 4:1 (w/w) with 93.74% yield. After complexation of saturated and less unsaturated fatty acids by urea complexation, argentation chromatography resulted in an EPA of high purity (99.6%) with an overall recovery of 54.09% using 50% diethyl ether/n-hexane as eluting solvent. The peroxide (POV) and thiobarbituric acid (TBS) values were found to be highest (4.0 mequiv of O2/kg and 5.2 mg of malondialdehyde/kg, respectively) during urea fractionation at the higher crystallization temperature (6 degrees C) and higher urea/fatty acid ratio (4:1). Keywords: Sardine oil; eicosapentaenoic acid (EPA); fatty acid methyl esters (FAME); urea fractionation; argentation column chromatography.

Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography. Publishing Authors By Initials

K Chakraborty,RP Raj,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

PUBMED ID PMID:

MEDLINE DATE:

Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of agricultural and food chemistry

VOLUME: 55

Page Numbers: 7586-95

Journal Abbreviation: J. Agric. Food Chem.

ISSN: 0021-8561

DAY: 25

MONTH: 07

YEAR: 2007

Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374755

Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: chemistry

Chemical & Substance for Abstract: Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography. Information

Substance Name: Silver Nitrate

Registry Number: 7761-88-8

Grant and Affiliation Information for Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography.

AFFILIATION: Physiology Nutrition and Pathology Division, Central Marine Fisheries Research Institute, Ernakulam North P.O., P.B. No. 1603, Cochin-682018, Kerala, India. kajal_iari@yahoo.com

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Agric Food Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography Related Publications

• An unusual manifestation of Abrus precatorius poisoning: A report of two cases.
• Cardiopulmonary Stress Testing in Children With Sickle Cell Disease Who are on Long-term Erythrocytapheresis.
• Characterization of protein transacetylase from human placenta as a signaling molecule calreticulin using polyphenolic peracetates as the acetyl group donors.
• Coat protein-mediated resistance against an Indian isolate of the Cucumber mosaic virus subgroup IB in Nicotiana benthamiana.
• Comparative quantification of ingress of trypan blue into the anterior chamber after microcoaxial, standard coaxial, and bimanual phacoemulsification Randomized clinical trial.
• Dental surgeon and medical health.
• Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography.
• Elevation of serum riboflavin carrier protein in hepatocellular carcinoma.
• Genetic polymorphisms of the RAS-cytokine pathway and chronic kidney disease.
• Humane killing of nonhuman animals for disease control purposes.
• In Vivo Evidence for the Role of CD44s in Promoting Breast Cancer Metastasis to the Liver.
• Ingress of bacterial inoculum into the anterior chamber after bimanual and microcoaxial phacoemulsification in rabbits.
• Intervertebral disc: anatomy-physiology-pathophysiology-treatment.
• Intimate partner violence around the time of pregnancy: association with breastfeeding behavior.
• Mercaptoethylpyrazine promoted electrochemistry of redox protein and amperometric biosensing of uric acid.
• Mitral valve repair in infants and children.
• Modulation of pulmonary vascular smooth muscle cell phenotype in hypoxia: role of cGMP-dependent protein kinase.
• Nodular secondary syphilis mimicking cutaneous lymphoreticular process.
• Optical sensing of biomedically important polyionic drugs using nano-sized gold particles.
• Platelet-activating factor stimulates ovine foetal pulmonary vascular smooth muscle cell proliferation: role of nuclear factor-kappa B and cyclin-dependent kinases.
• Protective mechanisms of p53-p21-pRb proteins against DNA damage-induced cell death.
• Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins.
• Regulation of endothelial nitric oxide synthase: involvement of protein kinase G 1 beta, serine 116 phosphorylation and lipid structures.
• Renal Function Outcomes in Patients Treated for Renal Masses Smaller Than 4 cm by Ablative and Extirpative Techniques.
• Role of platelet-activating factor in pulmonary vascular remodeling associated with chronic high altitude hypoxia in ovine fetal lambs.
• Shipbreaking in the developing world: problems and prospects.
• Syphilis and Hepatitis B Co-infection among HIV-Infected, Sex-Trafficked Women and Girls, Nepal.
• Techniques used for the determination of blood volume.
• Topical Lomefloxacin versus Topical Chloramphenicol in the Treatment of Acute Bacterial Conjunctivitis.
• Topical beta blockers and serum lipoproteins.