EGF and TGF-alpha supplementation enhances development of cloned mouse embryos.

EGF and TGF-alpha supplementation enhances development of cloned mouse embryos. Research Abstract Details 

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EGF and TGF-alpha supplementation enhances development of cloned mouse embryos. Abstract Text:

Tedla D Dadi,Ming-Wen Li,K C Kent Lloyd,Tedla D Dadi,Ming-Wen Li,K C Kent Lloyd,

In this study, we sought to determine the extent to which mitogenic growth factors affect the survival and development of cloned mouse embryos in vitro. Cloned embryos derived by intracytoplasmic nuclear injection (ICNI) of cumulus cell nuclei into enucleated oocytes were incubated in culture media supplemented with EGF and/or TGF-alpha for 4 days. Compared to control, treatment with either growth factor significantly increased the blastocyst formation rate, the total number of cells per blastocyst, the cell ratio of the inner cell mass and the trophectoderm (ICM:TE ratio), and EGF-R protein expression in cloned embryos. In most instances these effects were enhanced in cloned embryos when EGF and TGF-alpha were combined. Although fewer blastocysts developed from cloned than from fertilized one-cell stage embryos, growth factor treatment appeared to have the greatest effect on cloned embryos. These results demonstrate that mitogenic growth factors significantly enhance survival and promote the preimplantation development of cloned mouse embryos.

EGF and TGF-alpha supplementation enhances development of cloned mouse embryos. Publishing Authors By Initials

TD Dadi,MW Li,KC Lloyd,TD Dadi,MW Li,KC Lloyd,

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EGF and TGF-alpha supplementation enhances development of cloned mouse embryos. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cloning and stem cells

VOLUME: 9

Page Numbers: 315-26

Journal Abbreviation: Cloning Stem Cells

ISSN: 1536-2302

DAY: 2

MONTH: 10

YEAR: 2007

EGF and TGF-alpha supplementation enhances development of cloned mouse embryos. Information

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LANGUAGE: eng

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Grant and Affiliation Information for EGF and TGF-alpha supplementation enhances development of cloned mouse embryos.

AFFILIATION: Center for Comparative Medicine, School of Veterinary, University of California-Davis, Davis, California 95616, USA. dtdadi@ucdavis.edu

Country: United States

AGENCY: United States NCRR

GRANT: U42 RR14905

ACRONYM: RR

MEDLINETA: Cloning Stem Cells

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