Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection.

Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection. Research Abstract Details 

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Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection. Abstract Text:

Akie Shinmen,Arata Honda,Mika Ohkawa,Michiko Hirose,Narumi Ogonuki,Misako Yuzuriha,Hiromi Miki,Keiji Mochida,Kimiko Inoue,Kuniya Abe,Masao Ito,Atsuo Ogura,

Recently, mice and embryonic stem (ES) cells with allelic polymorphisms have been used extensively in the field of genetics and developmental biology. In this study, we examined whether intersubspecific hybrid mice and ES cells with these genotypes can be efficiently produced by intracytoplasmic sperm injection (ICSI). Frozen-thawed spermatozoa from wild-derived strains, JF1 (Mus musculus molossinus), MSM (M. m. molossinus), HMI (M. m. castaneus), and SWN (M. m. spp.), were directly injected into mature oocytes from laboratory mice ([C57BL/6 x DBA2]F1; M. m. domesticus). The in vitro and in vivo developmental capacity of F1 embryos was not significantly different among the groups (P > 0.05), and term offspring were efficiently obtained in all groups (27%-34% of transferred embryos). However, the mean body and placental weights of the offspring differed significantly with genotype (P < 5 x 10(-10)), with the HMI hybrid greatest in both body and placental weights. In an application study using these F1 offspring, we analyzed their mitochondrial DNA using intersubspecific polymorphisms and found the consistent disappearance of sperm mitochondrial DNA in the F1 progeny. In a second series of experiments, we generated F1 blastocysts by injecting MSM spermatozoa into C57BL/6 oocytes and used them to generate hybrid ES cell lines. The ES cell lines were established at a high efficiency (9 lines from 20 blastocysts) and their allelic polymorphisms were confirmed. Thus, ICSI using cryopreserved spermatozoa allows the efficient and immediate production of a number of F1 hybrid mice and ES cell lines, which can be used for polymorphic analysis of mouse genetics.

Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection. Publishing Authors By Initials

A Shinmen,A Honda,M Ohkawa,M Hirose,N Ogonuki,M Yuzuriha,H Miki,K Mochida,K Inoue,K Abe,M Ito,A Ogura,

For similar urogenital system: genitalia: germ cells: spermatozoa research abstracts see: urogenital system: genitalia: germ cells: spermatozoa research

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Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular reproduction and development

VOLUME: 74

Page Numbers: 1081-8

Journal Abbreviation: Mol. Reprod. Dev.

ISSN: 1040-452X

DAY: 29

MONTH: Sep

YEAR: 2007

Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection. Information

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LANGUAGE: eng

NlmUniqueID: 8903333

Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection. Keywords Mesh Terms:

KEYWORDS: Spermatozoa

MESH TERMS: physiology

Chemical & Substance for Abstract: Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection. Information

Substance Name: DNA, Mitochondrial

Registry Number: 0

Grant and Affiliation Information for Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection.

AFFILIATION: RIKEN Bioresource Center, Tsukuba, Ibaraki, Japan.

Country: United States

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MEDLINETA: Mol Reprod Dev

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