Efficient in vitro amplification of chronic wasting disease PrPRES.

Efficient in vitro amplification of chronic wasting disease PrPRES. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Efficient in vitro amplification of chronic wasting disease PrPRES. Abstract Text:

Timothy D Kurt,Matthew R Perrott,Carol J Wilusz,Jeffrey Wilusz,Surachai Supattapone,Glenn C Telling,Mark D Zabel,Edward A Hoover,

Chronic wasting disease (CWD) of cervids is associated with conversion of the normal cervid prion protein, PrP(C), to a protease-resistant conformer, PrP(CWD). Here we report the use of both nondenaturing amplification and protein-misfolding cyclic amplification (PMCA) to amplify PrP(CWD) in vitro. Normal brains from deer, transgenic mice expressing cervid PrP(C) [Tg(cerPrP)1536 mice], and ferrets supported amplification. PMCA using normal Tg(cerPrP)1536 brains as the PrP(C) substrate produced >6.5 x 10(9)-fold amplification after six rounds. Highly efficient in vitro amplification of PrP(CWD) is a significant step toward detection of PrP(CWD) in the body fluids or excreta of CWD-susceptible species.

Efficient in vitro amplification of chronic wasting disease PrPRES. Publishing Authors By Initials

TD Kurt,MR Perrott,CJ Wilusz,J Wilusz,S Supattapone,GC Telling,MD Zabel,EA Hoover,

For similar prion diseases: wasting disease, chronic research abstracts see: prion diseases: wasting disease, chronic research

PUBMED ID PMID:

MEDLINE DATE:

Efficient in vitro amplification of chronic wasting disease PrPRES. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virology

VOLUME: 81

Page Numbers: 9605-8

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 6

MONTH: 06

YEAR: 2007

Efficient in vitro amplification of chronic wasting disease PrPRES. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

Efficient in vitro amplification of chronic wasting disease PrPRES. Keywords Mesh Terms:

KEYWORDS: Wasting Disease, Chronic

MESH TERMS: diagnosis

Chemical & Substance for Abstract: Efficient in vitro amplification of chronic wasting disease PrPRES. Information

Substance Name: Prions

Registry Number: 0

Grant and Affiliation Information for Efficient in vitro amplification of chronic wasting disease PrPRES.

AFFILIATION: Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R21 AI58979

ACRONYM: AI

MEDLINETA: J Virol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Efficient in vitro amplification of chronic wasting disease PrPRES Related Publications

• Aerosol dosimetry research needs.
• Bereavement, gender, and cyberspace: a content analysis of parents' memorials to their children.
• Breast cancer incidence, 1980-2006: combined roles of menopausal hormone therapy, screening mammography, and estrogen receptor status.
• Carcinoma of the epiglottis treated by surgical excision with operative technic.
• Community as a key to healing after the death of a child.
• Direct analysis of the extracellular proteome from two strains of Helicobacter pylori.
• Effect of stimulus bandwidth on auditory skills in normal-hearing and hearing-impaired children.
• Efficient in vitro amplification of chronic wasting disease PrPRES.
• Empowerment will improve patient safety.
• Etanercept therapy for severe plaque psoriasis in a patient who underwent a liver transplant.
• Exploring the underlying hormonal mechanisms of prenatal risk factors for breast cancer: a review and commentary.
• FOOD CONSERVATION AND THE WAR.
• High-risk benign breast lesions: current strategies in management.
• Identification and characterization of potential sources of worker exposure to carbon nanofibers during polymer composite laboratory operations.
• Look, no hands! Unconventional transcriptional activators in bacteria.
• Menstrual and reproductive characteristics of women whose mothers were exposed in utero to diethylstilbestrol (DES).
• Mortality in women given diethylstilbestrol during pregnancy.
• PUBLIC HEALTH ENGINEERING: SEWAGE SLUDEG.
• Patient safety and surgeons: why the resistance?
• Phorbol ester stimulation of RasGRP1 regulates the sodium-chloride cotransporter by a PKC-independent pathway.
• Preeclampsia risk in women exposed in utero to diethylstilbestrol.
• Prenatal diethylstilbestrol exposure and risk of breast cancer.
• Shuttle lock release modification of a trans-tibial prosthesis with an obese client.
• Solar ultraviolet irradiation of human cornea, lens, and retina: equations of ocular irradiation.
• Surgeon Eddie L. Hoover, MD: Editor-in-Chief of the Journal of the National Medical Association from 2004 to present. Interview by George Dawson.
• The defining role of structure (including epitaxy) in the plausibility of homeopathy.
• Use of nisin-coated plastic films to control Listeria monocytogenes on vacuum-packaged cold-smoked salmon.
• Use of soy proteins in baked foods.
• Use of soy proteins in bakery products.
• Youth and chemical dependence.