Efficient gene delivery into mammalian cells mediated by a recombinant baculovirus containing a whispovirus ie1 promoter, a novel shuttle promoter between insect cells and mammalian cells.

Efficient gene delivery into mammalian cells mediated by a recombinant baculovirus containing a whispovirus ie1 promoter, a novel shuttle promoter between insect cells and mammalian cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Efficient gene delivery into mammalian cells mediated by a recombinant baculovirus containing a whispovirus ie1 promoter, a novel shuttle promoter between insect cells and mammalian cells. Abstract Text:

Hui Gao,Yu Wang,Na Li,Wu-Ping Peng,Yuan Sun,Guang-Zhi Tong,Hua-Ji Qiu,

Recombinant baculoviruses have emerged as a new gene delivery vehicle for mammalian cells. Thus, a shuttle promoter that mediates gene expression in both insect and mammalian cells will facilitate the development of a baculovirus vector-based mammalian cell gene delivery vehicle. This study described the generation of three recombinant baculoviruses with an EGFP reporter gene under the control of the white spot syndrome virus (WSSV) ie1 promoter, or either of two control promoters, the baculovirus early-to-late (ETL) promoter and polyhedrin promoter. The resulting recombinant baculoviruses were used to infect insect Sf9 cells and transduce several mammalian cell lines to test the expression of EGFP. We found that the WSSV ie1 promoter displayed a strong promoter activity in both insect and mammalian cells, and showed a stronger promoter activity than the ETL promoter in some mammalian cell lines. The activity of the WSSV ie1 promoter, but not the ETL promoter, can be enhanced by sodium butyrate, a histone deacetylase inhibitor. A transient plasmid transfection assay indicated that the WSSV ie1 promoter activity in mammalian cells is independent of baculovirus gene expression, differing from the ETL promoter, which was shown to be baculovirus-dependent. This study demonstrates, for the first time, that the WSSV ie1 promoter can function as a baculovirus-independent shuttle promoter between insect cells and mammalian cells. This novel shuttle promoter will facilitate the application of baculovirus-based vectors in gene expression, gene therapy, and non-replicative vector vaccines.

Efficient gene delivery into mammalian cells mediated by a recombinant baculovirus containing a whispovirus ie1 promoter, a novel shuttle promoter between insect cells and mammalian cells. Publishing Authors By Initials

H Gao,Y Wang,N Li,WP Peng,Y Sun,GZ Tong,HJ Qiu,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

PUBMED ID PMID:

MEDLINE DATE:

Efficient gene delivery into mammalian cells mediated by a recombinant baculovirus containing a whispovirus ie1 promoter, a novel shuttle promoter between insect cells and mammalian cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biotechnology

VOLUME: 131

Page Numbers: 138-43

Journal Abbreviation: J. Biotechnol.

ISSN: 0168-1656

DAY: 19

MONTH: 06

YEAR: 2007

Efficient gene delivery into mammalian cells mediated by a recombinant baculovirus containing a whispovirus ie1 promoter, a novel shuttle promoter between insect cells and mammalian cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8411927

Efficient gene delivery into mammalian cells mediated by a recombinant baculovirus containing a whispovirus ie1 promoter, a novel shuttle promoter between insect cells and mammalian cells. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Efficient gene delivery into mammalian cells mediated by a recombinant baculovirus containing a whispovirus ie1 promoter, a novel shuttle promoter between insect cells and mammalian cells. Information

Substance Name: Viral Proteins

Registry Number: 0

Grant and Affiliation Information for Efficient gene delivery into mammalian cells mediated by a recombinant baculovirus containing a whispovirus ie1 promoter, a novel shuttle promoter between insect cells and mammalian cells.

AFFILIATION: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, China.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biotechnol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Efficient gene delivery into mammalian cells mediated by a recombinant baculovirus containing a whispovirus ie1 promoter, a novel shuttle promoter between insect cells and mammalian cells Related Publications

• Frequency of loss expression of the DPC4 protein in various locations of biliary tract carcinoma.
• [A1166C polymorphism of the angiotensin II type 1 receptor gene and essential hypertension in Han, Tibetan and Yi populations]
• [Application of electro-acupuncture-anesthesia device in brachiplex nerve blocking]
• [Application of recombinant human activated protein C with blood product administration and deep vein thrombosis in severe sepsis]
• [Cause and mortality analysis of acute intracranial hypertension and cerebral edema in pediatric intensive care unit]
• [Clinical analysis on 21 cases of enterobiasis in nasal cavity]
• [Cloning and expression of two garlic virus coat protein genes]
• [Comparison of the effectiveness of chemotherapy and autologous hematopoietic stem cell transplantation as postremission treatment for adult acute lymphoblastic leukemia patients]
• [Determination of As, Hg and Mo in niuhuang jiedu tablets and other Chinese traditional medicines with graphite furnace AAS]
• [Determination of lead in tinplate by ICP-AES]
• [Enrichment and determination of trace chlorobenzene compounds in water]
• [In vivo degradation and tissue compatibility of polyphosphazene blend films]
• [Mechanical ventilation support for acute respiratory distress syndrome induced by severe sepsis and septic shock.]
• [Meta-analysis on curative effects of surgical procedures for intrahepatic bile duct lithiasis]
• [Pericardial devascularization with splenectomy for the treatment of portal hypertension]
• [Rapid determination of allethrin, permethrin and piperonyl butoxide in insecticidal aerosol for household by gas chromatography]
• [Role of the B lymphocytes in children with infectious mononucleosis caused by Epstein-Barr Virus]
• [Simultaneous determination of phenol and aniline in waste water by absorbance ratio derivative method]
• [Spectral diagnosis of dielectric barrier plasma discharge at atmospheric pressure and its application to surface modification of materials]
• [Studies on difference of fluorescence activity and elution order of propranolol enantiomers by reversed-phase high performance liquid chromatography]
• [Study on occupational respiratory diseases in fur-processing workers]
• [The determination of olaquindox in feeds by HPLC]
• [The expression of CD133 in acute leukemia and its clinical significance]
• [The identification of cortex from Kalopanax septemlobus and its confusions]
• [The inhibition pathway of the EBV-immortalized cells in children with infectious mononucleosis]
• [Therapeutic strategy of pediatric severe sepsis.]
• [Timing of surgical treatment for gallstone pancreatitis]
• [Trace analysis of Zn-doped compound semiconductor material by resonant ionization mass spectrometry]
• [Treatment with sedation and blood sugar control on severe sepsis.]
• [Trend of the CD23+ B cells in children with infectious mononucleosis caused by Epstein-Barr virus]