Efficient folding of the insect neuropeptide eclosion hormone by protein disulfide isomerase.

Efficient folding of the insect neuropeptide eclosion hormone by protein disulfide isomerase. Research Abstract Details 

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Efficient folding of the insect neuropeptide eclosion hormone by protein disulfide isomerase. Abstract Text:

N Fujita,M Yoshida,

Eclosion hormone is an insect neuropeptide that consists of 62 amino acid residues including three disulfide bonds. We have previously reported its hypothetical 3D structure consisting mainly of three alpha-helices. In this paper, we report the effects of chaperone proteins on the refolding of denatured eclosion hormone in a redox buffer containing reduced and oxidized glutathione. Urea-denatured eclosion hormone was spontaneously reactivated within 1 min with a yield of more than 90%, while beta-mercaptoethanol-denatured eclosion hormone was reactivated in a few minutes with a yield of 75%. Under the same experimental conditions, eclosion hormone treated with beta-mercaptoethanol and urea was reactivated slowly with a yield of 47% over a period of 2 h. Protein disulfide isomerase, a eucaryotic chaperone protein, markedly increased the reactivation yield and rate of the totally denatured hormone. GroE oligomers slightly improved the reactivation yield but peptidyl prolyl isomerase had no influence on yield or rate. We propose that the folding pathway of eclosion hormone involves at least two rate-limiting steps, and that protein disulfide isomerase is likely to be involved in the folding in insect neuronal cells.

Efficient folding of the insect neuropeptide eclosion hormone by protein disulfide isomerase. Publishing Authors By Initials

N Fujita,M Yoshida,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Efficient folding of the insect neuropeptide eclosion hormone by protein disulfide isomerase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 130

Page Numbers: 575-80

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 2001

Efficient folding of the insect neuropeptide eclosion hormone by protein disulfide isomerase. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Efficient folding of the insect neuropeptide eclosion hormone by protein disulfide isomerase. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Efficient folding of the insect neuropeptide eclosion hormone by protein disulfide isomerase. Information

Substance Name: Protein Disulfide-Isomerase

Registry Number: EC 5.3.4.1

Grant and Affiliation Information for Efficient folding of the insect neuropeptide eclosion hormone by protein disulfide isomerase.

AFFILIATION: Department of Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Noji-higashi, Kusatsu 525-8577, Japan. nori@se.ritsumei.ac.jp

Country: Japan

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MEDLINETA: J Biochem

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