Sialyl Tn (STn) and sialyl lactoside derivatives containing O-acetylated sialic acid residues have been chemoenzymatically synthesized using a one-pot three-enzyme system and conjugated to biotinylated human serum albumin (HSA) using an adipic acid para-nitrophenyl ester coupling reagent. This approach provides an efficient and general protocol for preparing carbohydrate-protein conjugates containing base-sensitive groups.
Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids. Publishing Authors By Initials
Chemical & Substance for Abstract: Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids. Information
Substance Name: Sialyltransferases
Registry Number: EC 2.4.99.-
Grant and Affiliation Information for Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids.
AFFILIATION: Department of Chemistry, University of California-Davis, One Shields Avenue, Davis, CA 95616, USA.
Country: England
AGENCY: United States NIGMS
GRANT: R01 GM 076360
ACRONYM: GM
MEDLINETA: Org Biomol Chem
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