Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector.

Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector. Research Abstract Details 

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Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector. Abstract Text:

Hajime Tsuda,Takuro Wada,Yoshinori Ito,Hiroaki Uchida,Hironari Dehari,Kiminori Nakamura,Katsunori Sasaki,Masayoshi Kobune,Toshihiko Yamashita,Hirofumi Hamada,

Strategies using mesenchymal stem cell (MSC)-mediated gene therapy have been developed to improve bone healing. However, transduction efficiency into MSCs by each vector is not always high. To overcome this problem, we used a modified adenoviral vector (Adv-F/RGD) with an RGD-containing peptide in the HI loop of the fiber knob domain of adenovirus type 5 (Ad5). Transduction efficiency into bone marrow-derived MSCs with Adv-F/RGD increased 12-fold compared with a vector containing the wild-type fiber (Adv-F/wt) by beta-galactosidase chemiluminescent assay. As a next step, we constructed AxCAhBMP2-F/RGD and AxCAhBMP2-F/wt carrying human bone morphogenetic protein 2 (BMP2). At the same multiplicity of infection, MSCs infected with AxCAhBMP2-F/RGD produced higher amounts of BMP2 than cells infected with AxCAhBMP2-F/wt, and also differentiated towards the osteogenic lineage more efficiently in vitro. Furthermore, using ex vivo gene transduction, we evaluated the potential for ectopic bone formation by the transduced MSCs in vivo. Transduction with AxCAhBMP2-F/RGD exhibited greatly enhanced new bone formation. These data suggest that Adv-F/RGD is useful for introducing foreign genes into MSCs and that it will be a powerful gene therapy tool for bone regeneration and other tissue engineering.

Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector. Publishing Authors By Initials

H Tsuda,T Wada,Y Ito,H Uchida,H Dehari,K Nakamura,K Sasaki,M Kobune,T Yamashita,H Hamada,

For similar genetic structures: genome: genome components: genes: transgenes research abstracts see: genetic structures: genome: genome components: genes: transgenes research

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Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular therapy : the journal of the American So

VOLUME: 7

Page Numbers: 354-65

Journal Abbreviation: Mol. Ther.

ISSN: 1525-0016

DAY: 19

MONTH: Mar

YEAR: 2003

Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector. Information

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LANGUAGE: eng

NlmUniqueID: 100890581

Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector. Keywords Mesh Terms:

KEYWORDS: Transgenes

MESH TERMS: physiology

Chemical & Substance for Abstract: Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector. Information

Substance Name: arginyl-glycyl-aspartic acid

Registry Number: 99896-85-2

Grant and Affiliation Information for Efficient BMP2 gene transfer and bone formation of mesenchymal stem cells by a fiber-mutant adenoviral vector.

AFFILIATION: Department of Molecular Medicine, Sapporo Medical University, Japan.

Country: United States

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MEDLINETA: Mol Ther

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