Efficient antibody production upon suppression of O mannosylation in the yeast Ogataea minuta.

Efficient antibody production upon suppression of O mannosylation in the yeast Ogataea minuta. Research Abstract Details 

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Efficient antibody production upon suppression of O mannosylation in the yeast Ogataea minuta. Abstract Text:

Kousuke Kuroda,Kazuo Kobayashi,Yoshinori Kitagawa,Taishiro Nakagawa,Haruhiko Tsumura,Toshihiro Komeda,Daisuke Shinmi,Eiji Mori,Kazuhiro Motoki,Kazumi Fuju,Teruyuki Sakai,Koichi Nonaka,Takeshi Suzuki,Kimihisa Ichikawa,Yasunori Chiba,Yoshifumi Jigami,Kousuke Kuroda,Kazuo Kobayashi,Yoshinori Kitagawa,Taishiro Nakagawa,Haruhiko Tsumura,Toshihiro Komeda,Daisuke Shinmi,Eiji Mori,Kazuhiro Motoki,Kazumi Fuju,Teruyuki Sakai,Koichi Nonaka,Takeshi Suzuki,Kimihisa Ichikawa,Yasunori Chiba,Yoshifumi Jigami,

When antibodies were expressed in the methylotrophic yeast Ogataea minuta, we found that abnormal O mannosylation occurred in the secreted antibody. Yeast-specific O mannosylation is initiated by the addition of mannose at serine (Ser) or threonine (Thr) residues in the endoplasmic reticulum via protein O mannosyltransferase (Pmt) activity. To suppress the addition of O-linked sugar chains on antibodies, we examined the possibility of inhibiting Pmt activity by the addition of a Pmt inhibitor during cultivation. The Pmt inhibitor was found to partially suppress the O mannosylation on the antibodies. Surprisingly, the suppression of O mannosylation was associated with an increased amount of assembled antibody (H2L2) and enhanced the antigen-binding activity of the secreted antibody. In this study, we demonstrated the expression of human antibody in O. minuta and elucidated the relationship between O mannosylation and antibody production in yeast.

Efficient antibody production upon suppression of O mannosylation in the yeast Ogataea minuta. Publishing Authors By Initials

K Kuroda,K Kobayashi,Y Kitagawa,T Nakagawa,H Tsumura,T Komeda,D Shinmi,E Mori,K Motoki,K Fuju,T Sakai,K Nonaka,T Suzuki,K Ichikawa,Y Chiba,Y Jigami,K Kuroda,K Kobayashi,Y Kitagawa,T Nakagawa,H Tsumura,T Komeda,D Shinmi,E Mori,K Motoki,K Fuju,T Sakai,K Nonaka,T Suzuki,K Ichikawa,Y Chiba,Y Jigami,

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Efficient antibody production upon suppression of O mannosylation in the yeast Ogataea minuta. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Applied and environmental microbiology

VOLUME: 74

Page Numbers: 446-53

Journal Abbreviation: Appl. Environ. Microbiol.

ISSN: 1098-5336

DAY: 26

MONTH: 11

YEAR: 2007

Efficient antibody production upon suppression of O mannosylation in the yeast Ogataea minuta. Information

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LANGUAGE: eng

NlmUniqueID: 7605801

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AFFILIATION: National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

Country: United States

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MEDLINETA: Appl Environ Microbiol

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