Effects of various electric fields on the fusion and in vitro development of mouse two-cell embryos.

Effects of various electric fields on the fusion and in vitro development of mouse two-cell embryos. Research Abstract Details 

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Effects of various electric fields on the fusion and in vitro development of mouse two-cell embryos. Abstract Text:

H T Cheong,T Taniguchi,M Hishinuma,Y Takahashi,H Kanagawa,

This study was undertaken to examine the effects of various electric fields such as alternating current (a.c.) voltage, fusion pulse strength, pulse duration, pulse number and electrode geometry on blastomere fusion and developmental rates of mouse two-cell embryos. The a.c. voltages (6 and 12 V/mm) did not affect the fusion and developmental rates. High fusion and developmental rates were obtained when pulse strengths of 1.0 to 2.5 kV/cm, pulse durations of 30 to 90 mu sec and pulse numbers of 1 to 6 were applied using a wire chamber. Comparison of electrode geometries showed that fusion rates were similarly high (93 to 98%) when pulse strengths of 1.0 to 2.5 kV/cm were applied, regardless of the electrode geometry. However, significantly lower developmental rates were observed in a rectangular chamber compared with those in a wire chamber, except when the pulse strength was 1.0 kV/cm. It was further observed that in a rectangular chamber, the developmental rate decreased with increasing pulse strength from 1.0 to 2.0 and 2.5 kV/cm. The results of this study indicate that by using a wire chamber, electric fields can be successfully applied across a relatively wide range of pulse strength, duration and number to provide sufficiently high fusion and subsequent developmental rates. The fusion conditions did, however, vary with chambers of different electrode geometries.

Effects of various electric fields on the fusion and in vitro development of mouse two-cell embryos. Publishing Authors By Initials

HT Cheong,T Taniguchi,M Hishinuma,Y Takahashi,H Kanagawa,

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Effects of various electric fields on the fusion and in vitro development of mouse two-cell embryos. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 36

Page Numbers: 875-85

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Nov

YEAR: 1991

Effects of various electric fields on the fusion and in vitro development of mouse two-cell embryos. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Effects of various electric fields on the fusion and in vitro development of mouse two-cell embryos.

AFFILIATION: Department of Theriogenology Faculty of Veterinary Medicine Hokkaido University, Sapporo 060, Japan.

Country: United States

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MEDLINETA: Theriogenology

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