Effects of ultraviolet B exposure on the expression of proliferating cell nuclear antigen in murine skin.

Effects of ultraviolet B exposure on the expression of proliferating cell nuclear antigen in murine skin. Research Abstract Details 

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Effects of ultraviolet B exposure on the expression of proliferating cell nuclear antigen in murine skin. Abstract Text:

Julian O Moore,Sapna R Palep,Rao N Saladi,Dayuan Gao,Yongyin Wang,Robert G Phelps,Mark G Lebwohl,Huachen Wei,

Proliferating cell nuclear antigen (PCNA) is an active nuclear protein involved in DNA replication, recombination and repair. PCNA is found throughout the basal layer in normal skin and in all layers of the epidermis in malignancy. This study evaluates PCNA's expression after acute and chronic UV-B irradiation. Skh-1 hairless mice exposed to 1.5 and 4.5 kJ/m2 of UV-B were sacrificed at 6, 12, 24, 48, 72 and 168 h. Immunohistochemical analysis revealed PCNA expression throughout the basal layer of untreated skin, with diminished expression at 6 h, indicative of immediate UV damage, and evidenced by the observable upregulation in pyrimidine dimer formation early on. Subsequently, PCNA immunoreactivity progressively increased, demonstrating an aberrant upward epidermal migratory pattern in association with chronic exposure. The 4.5 kJ/m2 group exhibited prolonged recovery in staining and also demonstrated this altered migratory pattern with chronic exposure. Progressive reactivation of PCNA expression occurs with repair. PCNA migration to upper layers of the epidermis indicates proliferation and possibly a subsequent increased malignant potential. We conclude that PCNA can serve as a marker of DNA repair and indirectly as an indicator of UV-B-induced damage, expression being time dependent and dose related. Specific immunoreactivity patterns and the observable atypia in keratinocytes are relevant in elucidating malignant potentiation.

Effects of ultraviolet B exposure on the expression of proliferating cell nuclear antigen in murine skin. Publishing Authors By Initials

JO Moore,SR Palep,RN Saladi,D Gao,Y Wang,RG Phelps,MG Lebwohl,H Wei,

For similar environment and public health: environment: meteorological factors: atmosphere: weather: sunlight: ultraviolet rays research abstracts see: environment and public health: environment: meteorological factors: atmosphere: weather: sunlight: ultraviolet rays research

PUBMED ID PMID:

MEDLINE DATE: 2004 Nov-Dec

Effects of ultraviolet B exposure on the expression of proliferating cell nuclear antigen in murine skin. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Photochemistry and photobiology

VOLUME: 80

Page Numbers: 587-95

Journal Abbreviation: Photochem. Photobiol.

ISSN: 0031-8655

DAY: 3

MONTH: 12

YEAR: 2007

Effects of ultraviolet B exposure on the expression of proliferating cell nuclear antigen in murine skin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376425

Effects of ultraviolet B exposure on the expression of proliferating cell nuclear antigen in murine skin. Keywords Mesh Terms:

KEYWORDS: Ultraviolet Rays

MESH TERMS: radiation effects

Chemical & Substance for Abstract: Effects of ultraviolet B exposure on the expression of proliferating cell nuclear antigen in murine skin. Information

Substance Name: Proliferating Cell Nuclear Antigen

Registry Number: 0

Grant and Affiliation Information for Effects of ultraviolet B exposure on the expression of proliferating cell nuclear antigen in murine skin.

AFFILIATION: Department of Dermatology, Mount Sinai School of Medicine, New York, NY 10029, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: R01 ES 09843

ACRONYM: ES

MEDLINETA: Photochem Photobiol

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