[Effects of traditional chinese medicine Drynaria fortunei smith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells]

[Effects of traditional chinese medicine Drynaria fortunei smith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells] Research Abstract Details 

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[Effects of traditional chinese medicine Drynaria fortunei smith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells] Abstract Text:

Qi Tang,Li-li Chen,Jie Yan,

OBJECTIVE: To explore the effects of Drynaria fortunei J. Smith (DFS) on promoting the proliferation, differentiation and calcification of cells. METHOD: Two DFS preparations were extracted with distilled water (DFS aqueous-extract) and 95% ethanol (DFS ethanol-extract), respectively. A mouse osteoblast cell line MC3T3-E1 was used as a cell model for screening potency of DFS. MTT and Flow cytometry were applied to determine proliferation of the cell promoted by DFS aqueous-and ethanol-extracts at different dosages. Differentiating effects of the two extracts with different concentrations in the cell were evaluated through the examinations of alkali phosphate (ALP) activities and osteocalcin levels. Von kossa staining method was used to understand the effects of the two extracts promoting calcification of the cell. RESULT: 0.01 mg x L(-1) and 1 mg x L(-1) of DFS aqueous-extracts showed the proliferative promotion for MC3T3-E1 cell (P < 0.05). Both the 0.01 mg x L(-1) and 1 mg x L(-1) of the two DFS extracts increased the percentages of S-phase cells whereas the percentages of G1-phase cells were decreased. 1 and 100 mg x L(-1) of the ethanol-extract remarkably increased the synthesis and secretion of osteocalcin of the cell (P < 0.001). 1 mg x L(-1) of the aqueous-extract and 0.01 mg x L(-1) of the ethanol-extract were able to promote the cell calcification (P < 0.05). CONCLUSION: In the DFS aqueous-and ethanol-extracts, there are some of the components promoting the proliferation, differentiation and calcification of osteoblast.

[Effects of traditional chinese medicine Drynaria fortunei smith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells] Publishing Authors By Initials

Q Tang,LL Chen,J Yan,

For similar plants: plant families and groups: ferns: polypodiaceae research abstracts see: plants: plant families and groups: ferns: polypodiaceae research

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[Effects of traditional chinese medicine Drynaria fortunei smith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazh

VOLUME: 29

Page Numbers: 164-8

Journal Abbreviation: Zhongguo Zhong Yao Za Zhi

ISSN: 1001-5302

DAY: 6

MONTH: Feb

YEAR: 2004

[Effects of traditional chinese medicine Drynaria fortunei smith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 8913656

[Effects of traditional chinese medicine Drynaria fortunei smith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells] Keywords Mesh Terms:

KEYWORDS: Polypodiaceae

MESH TERMS: chemistry

Chemical & Substance for Abstract: [Effects of traditional chinese medicine Drynaria fortunei smith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells] Information

Substance Name: Osteocalcin

Registry Number: 104982-03-8

Grant and Affiliation Information for [Effects of traditional chinese medicine Drynaria fortunei smith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells]

AFFILIATION: Department of Stomatology, the Second Affiliated Hospital College of Medical Science, Zhejiang University, Hangzhou, 310006, China.

Country: China

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MEDLINETA: Zhongguo Zhong Yao Za Zhi

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