[Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells]

[Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells] Abstract Text:

Jing He,Genze Shao,Rouli Zhou,

OBJECTIVE: To study the biological effects of the novel gene LAPTM4B high expressed in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells. METHODS: Eukaryotic expression plasmid pcDNA3-TM4B was constructed and transfected into mouse NIH3T3 cell line using Lipofectamin-2000 mediating gene transfer technique. Monoclonal transfected cells with high expression of LAPTM4B gene were identified and selected by RT-PCR, Northern blot and Western blot method. Cell surface morphology was detected by scanning electronic microscopy (SEM). The cell attachment/spreading on various matrices was examined. The cell growth curves were measured by acid phosphatase method. Cell cycle was detected by flow cytometry (FCM). The expression level of Cyclin E protein was examined using Western blot. RESULTS: The cell surface of the LAPTM4B transfected cells showed abundant tube/finger-like microvilli, which were distinguished significantly from the MOCK cells. The cell attachment/spreading of transfected cells increased on fibronectin, matrigel and laminin. The growth rate of transfected cells was faster than that of the MOCK cells. FCM analysis indicated that the amount of the transfected cells in S phase was increased significantly. Cyclin E protein expression of transfected cells was much higher than that of the MOCK cell. The serum-dependence of transfected cells was decreased. The fibrosarcoma was formed at a tumorigenic rate of 50% in NIH mice inoculated with LAPTM4B transfected cells. CONCLUSION: LAPTM4B gene promotes the cell proliferation by involving in the regulation of cell cycle control and causes tumorigenesis of NIH3T3 cells, indicating that it plays important roles in tumorigenesis.

[Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells] Publishing Authors By Initials

J He,G Shao,R Zhou,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Beijing da xue xue bao. Yi xue ban = Journal of Pe

VOLUME: 35

Page Numbers: 348-52

Journal Abbreviation: Beijing Da Xue Xue Bao

ISSN: 1671-167X

DAY: 1

MONTH: Aug

YEAR: 2003

[Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 101125284

[Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells]

AFFILIATION: Department of Cell Biology and Genetics, Peking University School of Basic Medical Sciences, Beijing 100083, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Beijing Da Xue Xue Bao

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells Related Publications

• Inhibitors of Na+/H+ and Na+/Ca2+ exchange depress intracellular calcium elevation induced by ischemia/reperfusion in rat cardiac myocytes.
• Inhibitory effect of recombinant FN polypeptide CH50 on the metastasis and tumor growth.
• Quantification of bcr/abl mRNA expression level in leukemia cells by FQ-RT-PCR.
• [An experimental study on the survival of the anterior abdominal skin flap after VEGF cDNA administration]
• [An inquiry into blood loss in laparoscopic liver resections]
• [Association studies of dopamine D4 receptor gene and dopamine transporter gene polymorphisms in Han Chinese patients with attention deficit hyperactivity disorder]
• [Beneficial effect of thyrotropin-releasing hormone in combination with HSD on hemorrhagic shock with pulmonary edema at high altitude in the rat]
• [Changes in platelet membrane glycoprotein levels in patients with cardiopulmonary resuscitation]
• [Clinical analysis of 13 infected total knee replacements]
• [Detection of epileptic waves in EEG based on wavelet transform]
• [Effects of Alexandrium tamarense and Prorocentrum donghaiense on rotifer Brachionus plicatilis population]
• [Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells]
• [Experimental study of K562 cell apoptosis induced by harringtonine]
• [Expression of otsA gene in tobacco and improvement stress tolerance]
• [Hemodynamic and pathologic changes in open abdominal wound after seawater immersion in dog]
• [High efficient expression of recombinant human platelet factor IV in pichia pastoris]
• [Identification of radix Rubiae and the root of Humulus scardens (Lour.) Merr.]
• [Incompatibility among indigenous plasmids of Sinorhizobium fredii strains and its application for plasmid curing]
• [Nutrition support of severe illness patients of general surgery during perioperation period]
• [Preliminary analysis of the characteristics of red tide areas in Changjiang River estuary and its adjacent sea]
• [Relationship between tissue inhibitors of metalloproteinase and metastasis and prognosis in breast cancer]
• [Repairing large alar defects by using a retro-grade auricular flap]
• [Research advance in effects of harmful algal bloom species on zooplankton]
• [Scoring protocol for quantitative diagnosis of gastrointestinal dysfunction and failure in severe cases]
• [Study on new techniques for genetic diagnosis of deletional alpha-thalassemia]
• [The influence of intrahepatic transplantation of hepatocytes and insular cells on liver cirrhosis]
• [The relationship of facet orientation to intervertebral disc protrusion and lateral recess stenosis in lower lumbar spine]
• [Transplantation of bone marrow cells upregulated vascular endothelial growth factor and its receptor and improved ischemic myocardial function]
• [Ultrasound surveillance of cervical lymph node metastasis in thoracic esophageal carcinoma]
• [Using scar tissue flap to repair secondary nasal deformities of unilateral cleft lip]