Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays.

Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays. Abstract Text:

Miranda Lo,Dieter M Bulach,David R Powell,David A Haake,James Matsunaga,Michael L Paustian,Richard L Zuerner,Ben Adler,

Leptospirosis is an important zoonosis of worldwide distribution. Humans become infected via exposure to pathogenic Leptospira spp. from infected animals or contaminated water or soil. The availability of genome sequences for Leptospira interrogans, serovars Lai and Copenhageni, has opened up opportunities to examine global transcription profiles using microarray technology. Temperature is a key environmental factor known to affect leptospiral protein expression. Leptospira spp. can grow in artificial media at a range of temperatures reflecting conditions found in the environment and the mammalian host. Therefore, transcriptional changes were compared between cultures grown at 20 degrees C, 30 degrees C, 37 degrees C, and 39 degrees C to represent ambient temperatures in the environment, growth under laboratory conditions, and temperatures in healthy and febrile hosts. Data from direct pairwise comparisons of the four temperatures were consolidated to examine transcriptional changes at two generalized biological conditions representing mammalian physiological temperatures (37 degrees C and 39 degrees C) versus environmental temperatures (20 degrees C and 30 degrees C). Additionally, cultures grown at 30 degrees C then shifted overnight to 37 degrees C were compared with those grown long-term at 30 degrees C and 37 degrees C to identify genes potentially expressed in the early stages of infection. Comparison of data sets from physiological versus environmental experiments with upshift experiments provided novel insights into possible transcriptional changes at different stages of infection. Changes included differential expression of chemotaxis and motility genes, signal transduction systems, and genes encoding proteins involved in alteration of the outer membrane. These findings indicate that temperature is an important factor regulating expression of proteins that facilitate invasion and establishment of disease.

Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays. Publishing Authors By Initials

M Lo,DM Bulach,DR Powell,DA Haake,J Matsunaga,ML Paustian,RL Zuerner,B Adler,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Infection and immunity

VOLUME: 74

Page Numbers: 5848-59

Journal Abbreviation:

ISSN: 0019-9567

DAY: 3

MONTH: Oct

YEAR: 2006

Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 246127

Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays.

AFFILIATION: Department of Microbiology, Monash University, Victoria 3800, Australia.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Infect Immun

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays Related Publications

• Anandamide and Delta9-tetrahydrocannabinol directly inhibit cells of the immune system via CB2 receptors.
• Beneficial effects of eplerenone versus hydrochlorothiazide on coronary circulatory function in patients with diabetes mellitus.
• Both early and late cognitive dysfunction affects the electroencephalogram in Parkinson's disease.
• Cancer treatment-induced bone loss.
• Cross-sectional analysis of specific complementary and alternative medicine (CAM) use by racial/ethnic group and menopausal status: the Study of Women's Health Across the Nation (SWAN).
• Cutaneous necrotizing vasculitis associated with hepatitis C virus infection.
• Defining mild cognitive impairment in Parkinson's disease.
• Dendritic cells program non-immunogenic prostate-specific T cell responses beginning at early stages of prostate tumorigenesis.
• Effective permeability of fractured porous media with power-law distribution of fracture sizes.
• Effects of follistatin overexpression on cell differentiation in the chick embryo retina.
• Effects of temperature on gene expression patterns in Leptospira interrogans serovar Lai as assessed by whole-genome microarrays.
• Enhanced expression of mucin genes in a guinea pig model of allergic asthma.
• Estradiol increases proteinuria and angiotensin II type 1 receptor in kidneys of rats receiving L-NAME and angiotensin II.
• Functional ability correlates with cognitive impairment in Parkinson's disease and Alzheimer's disease.
• Gambling and increased sexual desire with dopaminergic medications in restless legs syndrome.
• Histone acetylation at the Ifng promoter in tolerized CD4 cells is associated with increased IFN-gamma expression during subsequent immunization to the same antigen.
• Influence of the Stern layer on electrokinetic phenomena in porous media.
• Marked microglial reaction in normal aging human substantia nigra: correlation with extraneuronal neuromelanin pigment deposits.
• Mechanisms of T cell tolerance and suppression in cancer mediated by tumor-associated antigens and hormones.
• Mineralocorticoid receptor antagonist reduces renal injury in rodent models of types 1 and 2 diabetes mellitus.
• Nociceptin/orphanin FQ blocks the antinociception induced by mu, kappa and delta opioid agonists on the cold water tail-flick test.
• Perceived socioeconomic status: a new type of identity that influences adolescents' self-rated health.
• Pollinator and herbivore attraction to cucurbita floral volatiles.
• Steady state dendritic cells present parenchymal self-antigen and contribute to, but are not essential for, tolerization of naive and Th1 effector CD4 cells.
• Teaching resources. A biochemical assay for acetylcholinesterase activity in PC12 cells.
• The application of the HapMap to diabetic nephropathy and other causes of chronic renal failure.
• The chemokine CX3CL1/fractalkine interferes with the antinociceptive effect induced by opioid agonists in the periaqueductal grey of rats.
• The flare gene, which encodes the AIP1 protein of Drosophila, functions to regulate F-actin disassembly in pupal epidermal cells.
• Treatment of cavernous sinus tumors with linear accelerator radiosurgery.
• [Potential therapy for mucus hypersecretion in chronic obstructive pulmonary disease]