Effects of temperature and cholesterol on human erythrocyte membranes.

Effects of temperature and cholesterol on human erythrocyte membranes. Research Abstract Details 

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Effects of temperature and cholesterol on human erythrocyte membranes. Abstract Text:

T Yamaguchi,S Kuroki,M Tanaka,E Kimoto,

The effects of temperature and cholesterol on the membrane fluidity of human erythrocytes were studied using 5-nitroxide stearic acid (5NS), 12-nitroxide stearic acid (12NS), and 16-nitroxide stearic acid (16NS). Human erythrocytes and their lipid vesicles were treated in the range of 5--55 degrees C. In erythrocytes, ESR signals for 12NS and 16NS showed line broadening above 40 degrees C, whereas those for 5NS became sharper with increasing temperature as was the case with the signals of lipid vesicles for each label molecule. Lipid extraction from the heated sample caused no radical reduction. Only in 12NS-labeled erythrocytes did a weakly immobilized component and a strongly immobilized component appear. In the time course at 50 degrees C, the former decreased and the latter remained constant. From the ratio of both components, it was found that the interaction of the label molecules with the binding sites was determined by the physical state of the membrane. Furthermore, the dependence on temperature of the molecular motion of the labels in the cell membrane was irreversible above 40 degrees C. On addition of cholesterol to the membrane, the outer hyperfine splittings for 12NS and 16NS increased but that for 5NS decreased at C/P greater than 1, perhaps indicating a spread between the head groups of phospholipids by cholesterol.

Effects of temperature and cholesterol on human erythrocyte membranes. Publishing Authors By Initials

T Yamaguchi,S Kuroki,M Tanaka,E Kimoto,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

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Effects of temperature and cholesterol on human erythrocyte membranes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 92

Page Numbers: 673-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1982

Effects of temperature and cholesterol on human erythrocyte membranes. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Effects of temperature and cholesterol on human erythrocyte membranes. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: blood

Chemical & Substance for Abstract: Effects of temperature and cholesterol on human erythrocyte membranes. Information

Substance Name: Cholesterol

Registry Number: 57-88-5

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Country: JAPAN

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MEDLINETA: J Biochem

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