Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces.

Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces. Research Abstract Details 

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Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces. Abstract Text:

Li-Chong Xu,Christopher A Siedlecki,

Atomic force microscopy (AFM) was used to directly measure the adhesion forces between three test proteins and low density polyethylene (LDPE) surfaces treated by glow discharge plasma to yield various levels of water wettability. The adhesion of proteins to the LDPE substrates showed a step dependence on the wettability of surfaces as measured by the water contact angle (theta). For LDPE surfaces with theta> approximately 60-65 degrees , stronger adhesion forces were observed for bovine serum albumin, fibrinogen and human FXII than for the surfaces with theta<60 degrees . Smaller adhesion forces were observed for FXII than for the other two proteins on all surfaces although trends were identical. Increasing the contact time from 0 to 50s for each protein-surface combination increased the adhesion force regardless of surface wettability. Time varying adhesion data was fit to an exponential model and free energies of protein unfolding were calculated. This data, viewed in light of previously published studies, suggests a 2-step model of protein denaturation, an early stage on the order of seconds to minutes where the outer surface of the protein interacts with the substrate and a second stage involving movement of hydrophobic amino acids from the protein core to the protein/surface interface. Impact statement: The work described in this manuscript shows a stark transition between protein adherent and protein non-adherent materials in the range of water contact angles 60-65 degrees , consistent with known changes in protein adsorption and activity. Time-dependent changes in adhesion force were used to calculate unfolding energies relating to protein-surface interactions. This analysis provides justification for a 2-step model of protein denaturation on surfaces.

Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces. Publishing Authors By Initials

LC Xu,CA Siedlecki,

For similar natural sciences: chemistry: chemistry, physical: surface properties: wettability research abstracts see: natural sciences: chemistry: chemistry, physical: surface properties: wettability research

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Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biomaterials

VOLUME: 28

Page Numbers: 3273-83

Journal Abbreviation: Biomaterials

ISSN: 0142-9612

DAY: 12

MONTH: 04

YEAR: 2007

Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8100316

Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces. Keywords Mesh Terms:

KEYWORDS: Wettability

MESH TERMS: chemistry

Chemical & Substance for Abstract: Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces. Information

Substance Name: Polyethylene

Registry Number: 9002-88-4

Grant and Affiliation Information for Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces.

AFFILIATION: Department of Surgery, Biomedical Engineering Institute, The Pennsylvania State University, College of Medicine, Hershey, PA 17033, USA.

Country: England

AGENCY: United States NHLBI

GRANT: R01 HL69965

ACRONYM: HL

MEDLINETA: Biomaterials

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