Effects of stabilizers on the destabilization of proteins upon adsorption to aluminum salt adjuvants.

Effects of stabilizers on the destabilization of proteins upon adsorption to aluminum salt adjuvants. Research Abstract Details 

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Effects of stabilizers on the destabilization of proteins upon adsorption to aluminum salt adjuvants. Abstract Text:

Laura J Peek,Talia T Martin,Charity Elk Nation,Shannel A Pegram,C Russell Middaugh,

Excipients for protein-based vaccines are currently identified by evaluating the stability of the protein in solution. In most cases, however, the protein is adsorbed to the surface of an aluminum salt adjuvant in the final vaccine formulation. Previous studies showed that model protein antigens may be structurally altered and less thermally stable upon adsorption to aluminum salt adjuvants [Jones LS, Peek LJ, Power J, Markham A, Yazzie B, Middaugh CR, 2005, J Biol Chem 280:13406-13414]. The work presented herein provides evidence that compounds that stabilize the protein in solution also stabilize the adsorbed protein; however, the stability of the adsorbed protein in the presence of the stabilizer remains lower than that of the protein in solution. Potential implications of the reduced stability on the approach used to select excipients during formulation development are discussed.

Effects of stabilizers on the destabilization of proteins upon adsorption to aluminum salt adjuvants. Publishing Authors By Initials

LJ Peek,TT Martin,C Elk Nation,SA Pegram,CR Middaugh,

For similar complex mixtures: biological products: vaccines research abstracts see: complex mixtures: biological products: vaccines research

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Effects of stabilizers on the destabilization of proteins upon adsorption to aluminum salt adjuvants. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of pharmaceutical sciences

VOLUME: 96

Page Numbers: 547-57

Journal Abbreviation:

ISSN: 0022-3549

DAY: 3

MONTH: Mar

YEAR: 2007

Effects of stabilizers on the destabilization of proteins upon adsorption to aluminum salt adjuvants. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985195

Effects of stabilizers on the destabilization of proteins upon adsorption to aluminum salt adjuvants. Keywords Mesh Terms:

KEYWORDS: Vaccines

MESH TERMS: chemistry

Chemical & Substance for Abstract: Effects of stabilizers on the destabilization of proteins upon adsorption to aluminum salt adjuvants. Information

Substance Name: Vaccines

Registry Number: 0

Grant and Affiliation Information for Effects of stabilizers on the destabilization of proteins upon adsorption to aluminum salt adjuvants.

AFFILIATION: Department of Pharmaceutical Chemistry, University of Kansas, 2030 Becker Drive, Lawrence, Kansas 66047, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI-05421

ACRONYM: AI

MEDLINETA: J Pharm Sci

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