Effects of semen fractionation and dilution ratio on equine spermatozoal motility parameters.

Effects of semen fractionation and dilution ratio on equine spermatozoal motility parameters. Research Abstract Details 

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Effects of semen fractionation and dilution ratio on equine spermatozoal motility parameters. Abstract Text:

D D Varner,T L Blanchard,C L Love,M C Garcia,R M Kenney,

Two experiments were conducted to examine the effects of semen fractionation and dilution ratio on motility parameters of stallion spermatozoa. In Experiment 1, three ejaculates from each of three stallions were divided into sperm-rich (SR) and sperm-poor (SP) fractions to determine the difference in sperm concentration. Mean sperm concentration in SR fractions (349.5 x 10(6)/ml) was greater (P < 0.001) than that of SP fractions (96.9 x 10(6)/ml). In Experiment 2, three ejaculates from each of two stallions were divided into SR and SP fractions. Fifty percent of the original volume of SR fractions was combined with 50% of the original volume of SP fractions for each ejaculate to represent total ejaculates. SR and total ejaculates were diluted with skim milk-glucose semen extender as follows: 1) no dilution, or dilution to 2) 100 x 10(6)sperm/ml, 3) 50 x 10(6)sperm/ml, or 4) 25 x 10(6)sperm/ml. Semen samples were evaluated at 0.5, 3, 6, 12, and 24 h postejaculation (25 degrees C storage temperature) for percentages of total spermatozoal motility (TSM) and progressive spermatozoal motility (PSM). Mean TSM was greater (P < 0.05) in SR ejaculates than total ejaculates at 12 and 24 h postejaculation. Mean TSM of undiluted semen was lower (P < 0.05) than other dilution ratios over all periods. Mean TSM was greater (P < 0.05) at a 25 x 10(6)sperm/ml dilution ratio than a 50 x 10(6)sperm/ml dilution ratio at 12 and 24 h postejaculation, and greater (P < 0.05) than a 100 x 10(6)sperm/ml dilution ratio from 3 to 24 h postejaculation. Similar patterns were found for PSM. Collection of SR ejaculates and dilution to 25 x 10(6)sperm/ml improved longevity of spermatozoal motility.

Effects of semen fractionation and dilution ratio on equine spermatozoal motility parameters. Publishing Authors By Initials

DD Varner,TL Blanchard,CL Love,MC Garcia,RM Kenney,

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Effects of semen fractionation and dilution ratio on equine spermatozoal motility parameters. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 28

Page Numbers: 709-23

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Nov

YEAR: 1987

Effects of semen fractionation and dilution ratio on equine spermatozoal motility parameters. Information

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LANGUAGE: eng

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AFFILIATION: Department of Large Animal Medicine and Surgery, Texas Veterinary Medical Center, Texas A&M University, College Station, TX, USA.

Country: United States

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MEDLINETA: Theriogenology

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