[Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo]

[Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo] Research Abstract Details 

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[Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo] Abstract Text:

Xiang Zhang,Jingwen Wu,Xia Li,Luoan Fu,Dakuan Gao,Hongmin Bai,Xianzhen Liu,

OBJECTIVE: To investigate the effects of recombinant human bone morphogenic protein-2 (rhBMP-2) and hyaluronic acid (HA) on the invasion and metastasis of rat brain glioma in vivo. METHODS: C6 rat glioma cells were transfected with the plasmid vector named pEGFP-N3 which contained an enhanced green fluorescent protein (EGFP) gene. The stable cell clones which expressed EGFP protein were stereotactically injected into the brain parenchyma of SD rats in order to establish a xenotransplanted tumor model. After rhBMP-2 and HA were administered in the xenotransplanted tumor model at different dosages, their effects on glioma invasion and metastasis were observed by pathology, flow cytometry fluoresclnec, and electronmicvoscopy. RESULTS: EGFP-transfected C6 glioma cells gave off green fluorescence in vivo and in vitro. Area with tumor and area without tumor could be easily distinguished by fluorescence microscopy. Even distant micrometastasis and invasion at single-cell level could be detected. 10 microliter rhBMP-2 of the concentration of 5 microgram/ml inhibited C6 tumor invasion, while 10 microliter hyaluronic acid of the concentration of 100 microgram/ml evidently promoted the tumor invasion. CONCLUSION: EGFP-transfected C6 cells can be transplanted into rats' brain so as to establish an excellent experimental animal model in the research on invasion and metastasis of brain glioma in vivo. RhBMP-2 inhibits tumor invasion, while HA evidently promotes its invasion.

[Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo] Publishing Authors By Initials

X Zhang,J Wu,X Li,L Fu,D Gao,H Bai,X Liu,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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[Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua yi xue za zhi

VOLUME: 82

Page Numbers: 90-3

Journal Abbreviation: Zhonghua Yi Xue Za Zhi

ISSN: 0376-2491

DAY: 25

MONTH: Jan

YEAR: 2002

[Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 7511141

[Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo] Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: therapeutic use

Chemical & Substance for Abstract: [Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo] Information

Substance Name: Hyaluronic Acid

Registry Number: 9004-61-9

Grant and Affiliation Information for [Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo]

AFFILIATION: The Neurosurgical Institute of PLA, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China.

Country: China

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MEDLINETA: Zhonghua Yi Xue Za Zhi

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