Effects of recombinant human bone morphogenetic protein-2 on differentiation of cells isolated from human bone, muscle, and skin.

Effects of recombinant human bone morphogenetic protein-2 on differentiation of cells isolated from human bone, muscle, and skin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Effects of recombinant human bone morphogenetic protein-2 on differentiation of cells isolated from human bone, muscle, and skin. Abstract Text:

K Kawasaki,M Aihara,J Honmo,S Sakurai,Y Fujimaki,K Sakamoto,E Fujimaki,J M Wozney,A Yamaguchi,

We investigated the effects of recombinant human BMP-2 (rhBMP-2) on differentiation of cells isolated from human bone, muscle, and skin. Cells isolated from bones of six patients (HBM-1 to HBM-6), muscle from five patients (HM-1 to HM-5), and skin from three patients (HF-1 to HF-3) were used. rhBMP-2 had no effects on proliferation of two HBM cells, but had a stimulatory effect on three HM cell samples. rhBMP-2 stimulated both alkaline phosphatase (ALP) activity in all HBM cells and parathyroid hormone (PTH)-dependent cAMP production in three of the four HBM cell samples, although the magnitudes of these stimulatory effects differed among the cells tested. Although none of the HBM cells examined produced detectable amounts of osteocalcin in the absence of 1,25-(OH)2vitamin D3, they synthesized measurable amounts of osteocalcin in its presence. rhBMP-2 inhibited 1,25-(OH)2vitamin D3-dependent osteocalcin production in all of the HBM cell samples. Transplantation of HBM-6 cells with rhBMP-2 using diffusion chambers into the peritoneal cavity of athymic mice induced formation of cartilage and bone in the diffusion chambers, but neither cartilage nor bone was formed in chambers transplanted without rhBMP-2. rhBMP-2 also stimulated ALP activity in all of the HM and HF cell samples examined and PTH-dependent cAMP production in three of four HM cell samples. rhBMP-2 induced no osteocalcin production in any of the HM or HF cells in the presence of 1,25-(OH)2vitamin D3. rhBMP-2 markedly inhibited myotube formation by all of the HM cell samples. Transplantation of HM-4 cells with rhBMP-2, using diffusion chambers, into athymic mice induced ALP-positive cells in the chambers, but neither cartilage nor bone was observed. These results suggest that rhBMP-2 is a potent stimulator of osteoblast differentiation and bone formation in human cells.

Effects of recombinant human bone morphogenetic protein-2 on differentiation of cells isolated from human bone, muscle, and skin. Publishing Authors By Initials

K Kawasaki,M Aihara,J Honmo,S Sakurai,Y Fujimaki,K Sakamoto,E Fujimaki,JM Wozney,A Yamaguchi,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

Effects of recombinant human bone morphogenetic protein-2 on differentiation of cells isolated from human bone, muscle, and skin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Bone

VOLUME: 23

Page Numbers: 223-31

Journal Abbreviation: Bone

ISSN: 8756-3282

DAY: 19

MONTH: Sep

YEAR: 1998

Effects of recombinant human bone morphogenetic protein-2 on differentiation of cells isolated from human bone, muscle, and skin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8504048

Effects of recombinant human bone morphogenetic protein-2 on differentiation of cells isolated from human bone, muscle, and skin. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Effects of recombinant human bone morphogenetic protein-2 on differentiation of cells isolated from human bone, muscle, and skin. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Effects of recombinant human bone morphogenetic protein-2 on differentiation of cells isolated from human bone, muscle, and skin.

AFFILIATION: Department of Orthopedic Surgery, Showa University School of Medicine, Tokyo, Japan.

Country: UNITED STATES

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Bone

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Effects of recombinant human bone morphogenetic protein-2 on differentiation of cells isolated from human bone, muscle, and skin Related Publications

• A Model-based Numerical Analysis in the Early Development of Intracranial Aneurysms.
• A clinical study of 11 cases of combined hepatocellular-cholangiocarcinoma Assessment of enhancement patterns on dynamics computed tomography before resection.
• Adenovirus-Mediated MUC1 gene transduction into human blood-derived dendritic cells.
• Analysis of neurogenesis using transgenic mice expressing GFP with nestin gene regulatory regions.
• BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines.
• CCN3/NOV inhibits BMP-2-induced osteoblast differentiation by interacting with BMP and Notch signaling pathways.
• Changes in osteoblast phenotype during differentiation of enzymatically isolated rat calvaria cells.
• Correlation between the Appearance of Neuropeptides in the Rat Trigeminal Ganglion and Reinnervation of the Healing Root Socket after Tooth Extraction.
• Effect of free-radical scavengers and hyperbaric oxygen on random-pattern skin flaps.
• Effect of one-shot intravenous 0.42 mega unit urokinase therapy in patients with acute ischemic stroke.
• Effects of bone morphogenetic protein-2 and transforming growth factor-beta1 on gene expression of decorin and biglycan by cultured osteoblastic cells.
• Effects of recombinant human bone morphogenetic protein-2 on differentiation of cells isolated from human bone, muscle, and skin.
• Hydrodynamic interactions between two swimming bacteria.
• Intranuclear paracrystals observed in striated muscle specific LIM protein-deficient mouse cardiomyocytes.
• Isolation and characterization of a mesenchymal cell line that differentiates into osteoblasts in response to BMP-2 from calvariae of GFP transgenic mice.
• Low pathogenicity H5N2 avian influenza outbreak in Japan during the 2005-2006.
• Morphological study on quadruplets by cephalometric and model analyses.
• Netrin-1/DCC signaling in commissural axon guidance requires cell-autonomous expression of heparan sulfate.
• New insights into innate immunity in Arabidopsis.
• Nonequilibrium tricritical point in a system with long-range interactions.
• Nonverbal communication tool for children with severe motor and intellectual disabilities using biochemical measurement.
• Royal jelly prevents osteoporosis in rats: beneficial effects in ovariectomy model and in bone tissue culture model.
• Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP-2.
• The INTERPHONE study: design, epidemiological methods, and description of the study population.
• Three cases of ortho-phthalaldehyde-induced anaphylaxis after laryngoscopy: detection of specific IgE in serum.
• Transplantation of skin fibroblasts expressing BMP-2 contributes to the healing of critical-sized bone defects.
• Transplantation of skin fibroblasts expressing BMP-2 promotes bone repair more effectively than those expressing Runx2.
• Xenopus vocalizations are controlled by a sexually differentiated hindbrain central pattern generator.
• [Application of BMP to bone repair]
• [Quantitative study of osteoclastic related factors in the process of bone reconstruction]