Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver.

Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver. Abstract Text:

T Kawada,T Fujisawa,K Imai,K Ogata,

Employing livers from rats fed on a protein-free diet for two weeks, the effects of protein deficiency on both biosynthesis and degradation of rRNA were investigated and the following results were obtained. 1. Protein deficiency led to a decrease of total liver RNA content per DNA to about 80% of that in normal rat liver. 2. From the kinetics of rRNA labelling with [14C]orotic acid in vivo, the half-lives of cytoplasmic rRNA's of normal and protein-deficient rat livers were determined to be 6.2 and 5.1 days, respectively. Furthermore, considering the pool size of rRNA in rat liver, the turnover rate of cytoplasmic rRNA was calculated to be 0.212 pmole/min/mg of nuclear DNA in normal rats and 0.240 pmole/min/mg of nuclear DNA in protein-deficient rats. 3. From the electrophoretic patterns of nucleolar RNA's of both groups of rat livers labeled with [14C]orotic acid, the time courses of the specific activities of nucleolar 45S, 32S, and 28S rRNA's were analysed and the half-life of each nucleolar RNA in both groups of rat livers was determined. Nucleolar 45S, 32S, and 28S RNA's had half-lives of 6.0, 15.9, and 26.5 min in normal rats, respectively, and 5.5, 19.4, and 22.9 min in protein-deficient rats, respectively Considering the pool size of each nucleolar RNA obtained from the leectrophoretic pattern, the turnover rates of 45S, 32S, and 28S RNA's were calculated to be the same, i.e., o.189 pmoles/min/mg of nuclear DNA, in normal rat liver and 0.372, 0.372, and 0.358 pmoles/min/mg of nuclear DNA in protein-deficient rat liver, respectively. 4. These results indicate that protein deficiency increased both the rate of degradation of cytoplasmic rRNA and that of nucleolar rRNA synthesis in rat liver. While in normal rat liver the rates of rRNA synthesis and degradation were rather similar, the rate of rRNA synthesis in protein-deficient rats was about 1.5 times higher than that of its degradation. Therefore, the decrease of total liver RNA content in protein deficiency might be accounted for by stimulated degradation of rRNA in the nucleus. 5. The activities of RNase in nuclear fractions of both groups of rat livers were compared. Both activities of nuclear acid RNase and especially that of the free form of alkaline RNase in protein-deficient rat liver were higher than those in normal rat liver.

Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver. Publishing Authors By Initials

T Kawada,T Fujisawa,K Imai,K Ogata,

For similar enzymes and coenzymes: enzymes: hydrolases: esterases: ribonucleases research abstracts see: enzymes and coenzymes: enzymes: hydrolases: esterases: ribonucleases research

PUBMED ID PMID:

MEDLINE DATE:

Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 81

Page Numbers: 143-52

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1977

Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver. Keywords Mesh Terms:

KEYWORDS: Ribonucleases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver. Information

Substance Name: Ribonucleases

Registry Number: EC 3.1.-

Grant and Affiliation Information for Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver Related Publications

• Androgen receptor stimulates bone sialoprotein (BSP) gene transcription via cAMP response element and activator protein 1/glucocorticoid response elements.
• Biological process of carbohydrate attachment to ovalbumin.
• Complete cDNA sequence of the fourth component of murine complement.
• Constitutive expression of Slp genes in mouse strain B10.WR directed by C4 regulatory sequences.
• Effects of essential medicines on cardiovascular products available for the market in Thailand.
• Effects of protein deficiency on serum albumin-synthesizing activity of rat liver--with special reference to the presence of transcriptional controls.
• Effects of protein deficiency on the biosynthesis and degradation of ribosomal RNA in rat liver.
• Effects of the use of high concentrations of Mg2+ in the preparation of proteins from rat liver ribosomes pretreated with polyvinyl sulfate. Improved methods for preparing ribosomal proteins from ribosomal precursor particles of liver nucleoli.
• Evaluation of cleft lip bony depression of piriform margin and nasal deformity with cone beam computed tomography: "retruded-like" appearance and anteroposterior position of the alar base.
• Evidence for exclusive biosynthesis in vivo of serum albumin by bound polysomes of rat liver.
• Fermi-surface reconstruction without breakdown of Kondo screening at the quantum critical point.
• Hemoglobin biosynthesis in the chicken red blood cells and inhibitory effect of actinomycin D.
• Incorporation of 14C-amino acids into basic proteins of 47S and 32S subunits of rat liver ribosomes by a cell-free system.
• Isolation and identification of bovine tuberculosis in a Brazilian herd (São Paulo).
• Lack of exchange of ribosomal structural proteins with cell-sap proteins of rat liver in vitro.
• Malignant melanoma in a woman with LEOPARD syndrome: identification of a germline PTPN11 mutation and a somatic BRAF mutation.
• Mice constitutive for sex-limited protein (SLP) expression contain multiple Slp gene sequences.
• Preferential synthesis of arginase by free polysomes from rat liver.
• Purification and properties of acylamino acid-releasing enzyme from rat liver.
• Rat liver nucleolar 29.5S RNA as the precursor of nucleolar 28S RNA.
• Stabilizing effect of ribonuclease inhibitor on structure of polysomes and some properties of four classes of ribosomal particles in rat liver cytoplasm.
• Structure and expression of murine fourth complement component (C4) and sex-limited protein (Slp).
• Studies on SDS-phenol methods for extraction of rat liver nuclear RNA. I. Purity, recovery, and specific radioactivity of pulse labeled nuclear RNA obtained by SDS-phenol extraction under various conditions.
• Studies on SDS-phenol methods for extraction of rat liver nuclear RNA. II. Polyacrylamide gel electrophoresis of nuclear RNA obtained using various conditions for SDS-phenol extraction.
• Studies on creatine kinase of skeletal muscle and brain with special reference to subcellular distribution and isozymes.
• Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver.
• The different effects of N-ethylmaleimide and iodoacetamide on the activity of rat liver 60S subunits for peptide bond elongation.
• Transfected cDNA directs expression of hemolytically active murine C4 in cultured mouse and monkey cells.
• [Sudden cardiac death]
• cDNA sequence of a novel sex-limited protein (Slp) from mice constitutive for Slp expression. Sequence comparisons suggest that Slp has no functional role.