Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus.

Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus. Research Abstract Details 

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Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus. Abstract Text:

T Uzawa,A Yamagishi,T Ueda,N Chikazumi,K Watanabe,T Oshima,

A continuous cell-free protein synthesis system of an extremely thermophilic eubacterium, Thermus thermophilus HB27, was constructed. This system produced MS2 phage RNA translation products at a rate of more than 5 micrograms per hour per 1.9 mg of ribosomes at 65 degrees C and the production continued linearly for at least 340 min. When no polyamine was added, the system did not produce the proteins. The highest activity was recorded when 0.1 mM tetrakis(3-aminopropyl)ammonium and 1.0 mM spermine were added simultaneously.

Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus. Publishing Authors By Initials

T Uzawa,A Yamagishi,T Ueda,N Chikazumi,K Watanabe,T Oshima,

For similar bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus: thermus thermophilus research abstracts see: bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus: thermus thermophilus research

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Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 114

Page Numbers: 732-4

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1993

Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus. Keywords Mesh Terms:

KEYWORDS: Thermus thermophilus

MESH TERMS: metabolism

Chemical & Substance for Abstract: Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus. Information

Substance Name: Spermine

Registry Number: 71-44-3

Grant and Affiliation Information for Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus.

AFFILIATION: Department of Life Science, Tokyo Institute of Technology, Yokohama.

Country: JAPAN

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MEDLINETA: J Biochem

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