Effects of pH in Rapid-Equilibrium Enzyme Kinetics.

Effects of pH in Rapid-Equilibrium Enzyme Kinetics. Research Abstract Details 

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Effects of pH in Rapid-Equilibrium Enzyme Kinetics. Abstract Text:

Robert A Alberty,Robert A Alberty,Robert A Alberty,

The effects of pH on the rates of enzyme-catalyzed reactions are very important because they yield information on the pKs of acidic groups in the enzymatic site and the various enzyme-substrate complexes. But many enzyme-catalyzed reactions produce or consume hydrogen ions in a way that cannot be explained with pKs. These pH effects extend over the whole pH range of interest. In investigating these effects, the rapid-equilibrium assumption is especially useful because a large number of chemical reactions have to be taken into account. In these calculations, all of the reactions up to the rate-determining reaction are treated with biochemical thermodynamics. Kinetic studies make it possible to determine the number of hydrogen ions consumed in the rate-determining reaction, a number that can be in the range of 0-8. It is shown that the experimental limiting velocity of the forward reaction Vfexp is equal to 10npHVf, where n is a negative integer and Vf varies with pH in the way determined by the pKs of the enzyme-substrate complex that reacts in the rate-determining reaction. A computer program for the initial reaction velocity makes it possible to investigate the rapid-equilibrium kinetics of enzymatic mechanisms that involve the consumption of hydrogen ions.

Effects of pH in Rapid-Equilibrium Enzyme Kinetics. Publishing Authors By Initials

RA Alberty,RA Alberty,RA Alberty,

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Effects of pH in Rapid-Equilibrium Enzyme Kinetics. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The journal of physical chemistry. B

VOLUME: 111

Page Numbers: 14064-8

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ISSN: 1520-6106

DAY: 21

MONTH: 11

YEAR: 2007

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LANGUAGE: eng

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AFFILIATION: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.

Country: United States

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MEDLINETA: J Phys Chem B

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