Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea.

Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea. Research Abstract Details 

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Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea. Abstract Text:

Andrew R Reeves,Igor A Brikun,William H Cernota,Benjamin I Leach,Melissa C Gonzalez,J Mark Weber,

In carbohydrate-based fermentations of Saccharopolyspora erythraea, a polar knockout of the methylmalonyl-CoA mutase (MCM) gene, mutB, improved erythromycin production an average of 126% (within the range of 102-153% for a 0.95 confidence interval). In oil-based fermentations, where erythromycin production by the wild-type strain averages 184% higher (141-236%, 0.95 CI) than in carbohydrate-based fermentations, the same polar knockout in mutB surprisingly reduced erythromycin production by 66% (53-76%, 0.95 CI). A metabolic model is proposed where in carbohydrate-based fermentations MCM acts as a drain on the methylmalonyl-CoA metabolite pool, and in oil-based fermentations, MCM acts in the reverse direction to fill the methylmalonyl-CoA pool. Therefore, the model explains, in part, how the well-known oil-based process improvement for erythromycin production operates at the biochemical level; furthermore, it illustrates how the mutB erythromycin strain improvement mutation operates at the genetic level in carbohydrate-based fermentations.

Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea. Publishing Authors By Initials

AR Reeves,IA Brikun,WH Cernota,BI Leach,MC Gonzalez,JM Weber,

For similar cells: spores: spores, bacterial research abstracts see: cells: spores: spores, bacterial research

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Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of industrial microbiology & biotechnology

VOLUME: 33

Page Numbers: 600-9

Journal Abbreviation: J. Ind. Microbiol. Biotechnol.

ISSN: 1367-5435

DAY: 21

MONTH: 02

YEAR: 2006

Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9705544

Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea. Keywords Mesh Terms:

KEYWORDS: Spores, Bacterial

MESH TERMS: genetics

Chemical & Substance for Abstract: Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea. Information

Substance Name: Methylmalonyl-CoA Mutase

Registry Number: EC 5.4.99.2

Grant and Affiliation Information for Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea.

AFFILIATION: Fermalogic Inc., 2201 West Campbell Park Drive, Chicago, IL 60612, USA.

Country: England

AGENCY: United States NIGMS

GRANT: R44GM58943

ACRONYM: GM

MEDLINETA: J Ind Microbiol Biotechnol

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