Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy.

Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy. Research Abstract Details 

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Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy. Abstract Text:

Plasmid vector is increasingly applied to gene therapy or gene vaccine. The production of plasmid pCMV-AP3 for cancer gene therapy was conducted in a modified MBL medium using a recombinant E. coli BL21 system. The effects of different MMBL components on plasmid yield, cell mass and specific plasmid DNA productivity were evaluated on shake-flask scale. The results showed that glucose was the optimal carbon source. High plasmid yield (58.3 mg/L) was obtained when 5.0 g/L glucose was added to MMBL. Glycerol could be chosen as a complementary carbon source because of the highest specific plasmid productivity (37.9 mg DNA/g DCW). After tests of different levels of nitrogen source and inorganic phosphate, a modified MMBL medium was formulated for optimal plasmid production. Further study showed that the initial acetate addition (less than 4.0 g/L) in MMBL improved plasmid production significantly, although it inhibited cell growth. The results will be useful for large-scale plasmid production using recombinant E. coli system.

Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy. Publishing Authors By Initials

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

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Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of Zhejiang University. Science. B

VOLUME: 6

Page Numbers: 396-400

Journal Abbreviation:

ISSN: 1673-1581

DAY: 25

MONTH: May

YEAR: 2005

Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy. Information

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LANGUAGE: eng

NlmUniqueID: 101236535

Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: genetics

Chemical & Substance for Abstract: Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy.

AFFILIATION: Institute Bioengineering, School of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China. znxu@zju.edu.cn

Country: China

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MEDLINETA: J Zhejiang Univ Sci B

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