Effects of lipopolysaccharide on gene expression of antimicrobial peptides (penaeidins and crustin), serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei.

Effects of lipopolysaccharide on gene expression of antimicrobial peptides (penaeidins and crustin), serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Effects of lipopolysaccharide on gene expression of antimicrobial peptides (penaeidins and crustin), serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei. Abstract Text:

Takuji Okumura,

For shrimp immune defences, prophenoloxidase (proPO) activating system and antimicrobial peptides in circulating haemocytes play important roles. In the present study, the effects of lipopolysaccharide (LPS) injection on gene expression of penaeidins, crustin, serine proteinase and proPO in haemocytes were determined using real-time reverse transcription-polymerase chain reaction (PCR) in the Pacific white shrimp Litopenaeus vannamei. After injection of LPS, mRNA levels of antimicrobial peptides, penaeidin 2 (PEN2), penaeidin 3 (PEN3), penaeidin 4 (PEN4) and crustin decreased in a dose-dependent manner, while mRNA levels of serine proteinase and proPO did not change significantly. In a time-course experiment, injection of LPS caused significant depression in mRNA levels of PEN2, PEN3, PEN4 and crustin at 4h post-injection, and the depressed mRNA levels returned to initial levels by 72h post-injection. On the other hand, mRNA levels of serine proteinase and proPO did not show a significant change after injection. These results suggest that the antimicrobial peptide system responds to LPS injection at a gene expression level while the proPO system does not respond at a gene expression level.

Effects of lipopolysaccharide on gene expression of antimicrobial peptides (penaeidins and crustin), serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei. Publishing Authors By Initials

T Okumura,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Effects of lipopolysaccharide on gene expression of antimicrobial peptides (penaeidins and crustin), serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Fish & shellfish immunology

VOLUME: 22

Page Numbers: 68-76

Journal Abbreviation: Fish Shellfish Immunol.

ISSN: 1050-4648

DAY: 30

MONTH: 03

YEAR: 2006

Effects of lipopolysaccharide on gene expression of antimicrobial peptides (penaeidins and crustin), serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9505220

Effects of lipopolysaccharide on gene expression of antimicrobial peptides (penaeidins and crustin), serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Effects of lipopolysaccharide on gene expression of antimicrobial peptides (penaeidins and crustin), serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Effects of lipopolysaccharide on gene expression of antimicrobial peptides (penaeidins and crustin), serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei.

AFFILIATION: National Research Institute of Aquaculture, Fisheries Research Agency, Minami-ise, Mie 516-0193, Japan. takuji@affrc.go.jp

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Fish Shellfish Immunol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Effects of lipopolysaccharide on gene expression of antimicrobial peptides penaeidins and crustin, serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei Related Publications

• Aldosterone causes vasoconstriction in coronary arterioles of rats via angiotensin II type-1 receptor: influence of hypertension.
• Automated Discrimination of Tissue Boundaries using Ultrasound Images of "Ubiquitous Echo".
• Automatic anatomical labeling method of cerebral arteries in MR-angiography data set.
• Beneficial effect of neutrophil elastase inhibitor on anti-Thy1.1 nephritis in rats.
• Characterization of alternatively spliced isoforms of the type I interleukin-1 receptor on iNOS induction in rat hepatocytes.
• Chemosensitivity assessed by collagen gel droplet embedded culture drug sensitivity test, and MDR1, MRP1, and MRP2 mRNA expression in human colorectal adenocarcinomas.
• Determination of the absolute configuration of urothion.
• Effect of active hexose correlated compound on the production of nitric oxide in hepatocytes.
• Effect of thiol-containing molecule cysteamine on the induction of inducible nitric oxide synthase in hepatocytes.
• Effects of bilateral and unilateral eyestalk ablation on vitellogenin synthesis in immature female kuruma prawns, Marsupenaeus japonicus.
• Effects of lipopolysaccharide on gene expression of antimicrobial peptides (penaeidins and crustin), serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei.
• Expression of humanized Fab fragments that recognize the IgE-binding domain of human Fc(epsilon)RIalpha in COS and CHO cells.
• Glycaemic control and serum intact parathyroid hormone levels in diabetic patients on haemodialysis therapy.
• Impact of the high-molecular-weight form of adiponectin on endothelial function in healthy young men.
• Improvement in image quality of noncontrast head images in multidetector-row CT by volume helical scanning with a three-dimensional denoising filter.
• Interaction of alpha1-syntrophin with multiple isoforms of heterotrimeric G protein alpha subunits.
• Lipopolysaccharide induces adipose differentiation-related protein expression and lipid accumulation in the liver through inhibition of fatty acid oxidation in mice.
• MSPL/TMPRSS13.
• Microanalysis of action patterns of amylolytic enzymes: identification of a glucose-forming amylase in Japanese radish root.
• Na(+)/H(+) exchanger inhibitor, FR183998, has protective effect in lethal acute liver failure and prevents iNOS induction in rats.
• Nicorandil suppresses the increases in plasma level of matrix metalloproteinase activity and attenuates left ventricular remodeling in patients with acute myocardial infarction.
• Orotate as a beta-alanine donor for anserine and carnosine biosynthesis, and effects of actinomycin D and azauracil on their pathway.
• Pitavastatin up-regulates the induction of iNOS through enhanced stabilization of its mRNA in pro-inflammatory cytokine-stimulated hepatocytes.
• Rebamipide, anti-gastric ulcer drug, up-regulates the induction of iNOS in proinflammatory cytokine-stimulated hepatocytes.
• The effects of endothelial nitric oxide synthase gene polymorphisms on endothelial function and metabolic risk factors in healthy subjects: the significance of plasma adiponectin levels.
• Transition-metal-mediated cascade reactions: C,C-dicyclopropylmethylamines by way of double C,C-sigma-bond insertion into bicyclobutanes.
• [Arrhythmias in dialysis patients]
• [Gastric lavage]
• [Metabolic syndrome]
• [The role of a memory clinic in regional supporting system for mild dementia patients and caregivers]