[Effects of isoflurane on lung surfactant protein A in rats]

[Effects of isoflurane on lung surfactant protein A in rats] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Effects of isoflurane on lung surfactant protein A in rats] Abstract Text:

Zhi-hong Li,Ba-xian Yang,Hai-Yan An,

OBJECTIVE: To examine the effects of different concentrations of Isoflurane on the level of surfactant protein A(SP-A) and the expression of SP-A mRNA in the lung of rats. METHODS: Thirty-two male Wistar rats were randomly divided into 4 groups: control group received 40% (volume fraction) O2 inhalation (40% O2, n=8); 0.7% isoflurane group (n=8), 1.5% isoflurane group (n=8), and 2.0% isoflurane group (n=8). They were treated with 40% O2 with 0.7%, 1.5% and 2.0% isoflurane respectively. In each group, the rats inhaled experimental gas for 8 hours, and then were put to death immediately. Morphological changes of type II pneumocytes were observed by transmission electron microscopy. Surfactant protein content in broncho-alveolar lavage fluid (BALF) was measured with Western Blotting. The level of intracellular SP-A was examined with immunohistochemistry (IHC). To assess the relative levels of SP-A mRNA in lung tissue, reverse transcriptase-polymerase chain reaction (RT-PCR) was used with the co-amplification of the "housekeeping" gene GAPDH as internal control. RESULTS: Morphological changes of type II pneumocytes were distinct in 1.5% isoflurane and 2.0% isoflurane groups. Isoflurane, after three doses, significantly reduced the SP-A content in BALF. Control group, 437,112+/-25,654; 0.7% isoflurane group, 355,789+/-28,116; 1.5% isoflurane group, 238,554+/-31,531; 2.0% isoflurane group, 223,632+/-25,710 (P<0.01); Intracellular SP-A content of 1.5% and 2.0% isoflurane groups was less than that of the control, whereas 0.7% isoflurane groups did not show any significant difference from the control. RT-PCR indicated a reduced expression of SP-A mRNA in the lung after 1.5% and 2.0% isoflurane exposure. Control group, 1.47+/-0.18; 1.5% isoflurane group, 1.19+/-0.13; 2.0% isoflurane group, 1.13+/-0.12 (P<0.01). CONCLUSION: These findings indicate that 1.5% or more than 1.5% isoflurane exposure for eight hours could decrease the expression of the SP-A mRNA and synthesis of SP-A in rats, and maybe it could also affect the secretion and reuse.

[Effects of isoflurane on lung surfactant protein A in rats] Publishing Authors By Initials

ZH Li,BX Yang,HY An,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Effects of isoflurane on lung surfactant protein A in rats] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Beijing da xue xue bao. Yi xue ban = Journal of Pe

VOLUME: 38

Page Numbers: 402-6

Journal Abbreviation:

ISSN: 1671-167X

DAY: 18

MONTH: Aug

YEAR: 2006

[Effects of isoflurane on lung surfactant protein A in rats] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 101125284

[Effects of isoflurane on lung surfactant protein A in rats] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Effects of isoflurane on lung surfactant protein A in rats] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Effects of isoflurane on lung surfactant protein A in rats]

AFFILIATION: Department of Anesthesiology, Peking University People's Hospital, Beijing 100044, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Beijing Da Xue Xue Bao

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Effects of isoflurane on lung surfactant protein A in rats Related Publications

• Cytoskeleton dynamics: fluctuations within the network.
• Evaluation of 99mTc labeled diadenosine tetraphosphate as an atherosclerotic plaque imaging agent in experimental models.
• Evidence-based modeling of critical illness: an initial consensus from the Society for Complexity in Acute Illness.
• Integrating all medical records to an enterprise viewer.
• Intradiscal injections of osteogenic protein-1 restore the viscoelastic properties of degenerated intervertebral discs.
• Molecular genetic studies on relationships among longevity, diseases, and HLA-DRB1/DQB1 allelic polymorphism.
• Research advances on transgenic plant vaccines.
• Sagittal plane motion in the human lumbar spine: comparison of the in vitro quasistatic neutral zone and dynamic motion parameters.
• Sensory neurons and fibers from multiple spinal cord levels innervate the rabbit lumbar disc.
• The architecture of enterprise hospital information system.
• [Alcohol experience, alcohol knowledge, and alcohol expectancy in early adolescents]
• [Construction of fusion expression vector EGFP-PDX-1 and its transfection into rat fetal hepatic stem cells by electroporation]
• [Determination of polyether silicon oil by IR]
• [Effects of exercise before steroid treatment on type i and type ii hindlimb muscles in a rat model]
• [Effects of ginaton on the markers of myocardial injury during cardiopulmonary bypass]
• [Effects of isoflurane on lung surfactant protein A in rats]
• [Effects of isometric contraction training by electrostimulation on Type I and II hindlimb muscles in cerebral ischemia model rats]
• [Expression of caveolin-1 protein in the rat brain and its role in the discrimination learning]
• [Expression vector for the inhibitor of growth-1 gene is constructed and the NLS-GFP fusion protein expresses in MRC-5 cells]
• [Mechanisms of the expression of exogenous genes injected via the mammary duct in rat mammary gland tissue]
• [Progress of Tol2 transposable element in Medaka fish]
• [Progress of the PCR amplification techniques for chromosome walking]
• [SERS spectra of vitamin A acid in silver solution]
• [Status of nosocomial urinary tract infections in the ICU: molecular epidemiology of imipenem resistant P. aeruginosa]
• [Surgical treatment of naso-ethmoid-orbital fracture]
• [Synthesis of dominant epitopes on N-terminal part of BPI and preparation of corresponding antisera]
• [Target lethal effect of recombinant soluble Fas coupled with protein kinase C inhibitor on colorectal carcinoma cells]
• [Temperature effect on the noninvasive measurement of human blood glucose by NIR]
• [The progress on T-DNA integration research]
• [Volumetric measurement of human orbit by means of computed tomographic analysis based on CT scanning digital data]