Effects of HBV X gene and arsenic trioxide on the expression of p53 in cultured HepG2 cells.

Effects of HBV X gene and arsenic trioxide on the expression of p53 in cultured HepG2 cells. Research Abstract Details 

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Effects of HBV X gene and arsenic trioxide on the expression of p53 in cultured HepG2 cells. Abstract Text:

Jian-hua Lei,Xing-e He,Xu Yang,Min Zhang,Jun Lian,Hong-Yu Luo,Wen-long Wang,Jian-Hua Lei,Xing-E He,Xu Yang,Min Zhang,Jun Lian,Hong-Yu Luo,Wen-Long Wang,Jian-hua Lei,Xing-e He,Xu Yang,Min Zhang,Jun Lian,Hong-Yu Luo,Wen-long Wang,Jian-hua Lei,Xing-e He,Xu Yang,Min Zhang,Jun Lian,Hong-Yu Luo,Wen-long Wang,

BACKGROUND: Hepatitis B virus (HBV) X protein (HBx) and p53 could mutually down-regulate at transcriptional level and HBx could bind with p53 protein within its transactivation domain and inhibit the function of p53 protein. In recent years, effects of arsenic trioxide (As(2)O(3)) on the expression of p53 protein have been widely studied, while little is known about the activity of p53 protein. This study was undertaken to delineate the effect of HBV X gene and As(2)O(3) on p53 protein expression (level and activity) in HepG2 cells by small hairpin RNA (shRNA)-mediated RNA interference (RNAi) technique. METHODS: Cell line HepG2 and cells with stable expression of HBV X gene (HepG2-X) were treated with 2 micromol/L As(2)O(3), with corresponding untreated cells serving as controls. Cell lysates and nuclear extracts were extracted. Total level and the relative activity of p53 protein were detected by modified enzyme-linked immunosorbent assay (ELISA). HBV X gene sequence-specific shRNA expression vector (pXi-1 and pXi-2) and sequence-unrelated control (pXi-3) were transfected into HepG2-X. Single cell clone with stable expression of shRNA was selected and exposed to propagating culture. The effect of As(2)O(3) on p53 protein expression and activity was re-observed. RESULTS: Total p53 protein level was up-regulated and its relative activity ratio was enhanced by As(2)O(3) in HepG2 and HepG2-X cells. The total p53 protein level induced by As(2)O(3) was up-regulated by HBV X gene expression, while its relative activity was significantly suppressed. The suppression was removed after HBV X gene expression was repressed by shRNA. CONCLUSIONS: As(2)O(3) up-regulates p53 protein expression and enhance its activity. HBV X up-regulates As(2)O(3) induced-p53 protein expression while suppresses its activity.

Effects of HBV X gene and arsenic trioxide on the expression of p53 in cultured HepG2 cells. Publishing Authors By Initials

JH Lei,XE He,X Yang,M Zhang,J Lian,HY Luo,WL Wang,JH Lei,XE He,X Yang,M Zhang,J Lian,HY Luo,WL Wang,JH Lei,XE He,X Yang,M Zhang,J Lian,HY Luo,WL Wang,JH Lei,XE He,X Yang,M Zhang,J Lian,HY Luo,WL Wang,

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Effects of HBV X gene and arsenic trioxide on the expression of p53 in cultured HepG2 cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Chinese medical journal

VOLUME: 120

Page Numbers: 2181-4

Journal Abbreviation: Chin. Med. J.

ISSN: 0366-6999

DAY: 20

MONTH: Dec

YEAR: 2007

Effects of HBV X gene and arsenic trioxide on the expression of p53 in cultured HepG2 cells. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Effects of HBV X gene and arsenic trioxide on the expression of p53 in cultured HepG2 cells.

AFFILIATION: Liver Disease Research Center, Second Xiangya Hospital, Central South University, Changsha 410011, China.

Country: China

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MEDLINETA: Chin Med J (Engl)

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