Effects of growth factors on cell migration and alkaline phosphatase release.

Effects of growth factors on cell migration and alkaline phosphatase release. Research Abstract Details 

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Effects of growth factors on cell migration and alkaline phosphatase release. Abstract Text:

Krista N Gonnerman,Lauren S Brown,Tien-Min G Chu,Krista N Gonnerman,Lauren S Brown,Tien-Min G Chu,

Segmental defects in long bones pose a clinical challenge in orthopaedic surgery. These defects often require multiple surgeries to achieve the desired bridging and union. Bone morphogenetic protein 2 (BMP-2) has been used extensively to facilitate regeneration in these large defects. The two important roles that BMP-2 has to play in large defect regeneration are chemotaxis to bone cells to migrate and induction of bone matrix production. Experiments were designed to study the effect of BMP-2 on alkaline phosphatase (ALP) production in cells that have migrated through a porous membrane in a cell migration chamber under the influence of culture media supplemented with BMP-2. Two cell lines, UMR cells and MC3T3-E1 cells, were used. A modified Boyden chamber with 8 microm membrane filter was used. Cell migration under the influence of BMP-2 concentration of 1, 10, 100, and 1000 ng/ml was studied. In both cells, the highest chemotactic effect was observed at 10 ng/ml. The ALP activity in the migrated cell was then characterized. A pattern of increase in ALP activity with increased BMP-2 dose was observed in the migrated cells.

Effects of growth factors on cell migration and alkaline phosphatase release. Publishing Authors By Initials

KN Gonnerman,LS Brown,TM Chu,KN Gonnerman,LS Brown,TM Chu,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Effects of growth factors on cell migration and alkaline phosphatase release. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biomedical sciences instrumentation

VOLUME: 42

Page Numbers: 60-5

Journal Abbreviation:

ISSN: 0067-8856

DAY: 19

MONTH: 11

YEAR: 2006

Effects of growth factors on cell migration and alkaline phosphatase release. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 140524

Effects of growth factors on cell migration and alkaline phosphatase release. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: administration & dosage

Chemical & Substance for Abstract: Effects of growth factors on cell migration and alkaline phosphatase release. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Effects of growth factors on cell migration and alkaline phosphatase release.

AFFILIATION: Department of Biomedical Engineering, Indiana University-Purdue University Indianapolis, 1120 South Drive, Rm. 146, Indianapolis, IN 46202, USA.

Country: United States

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MEDLINETA: Biomed Sci Instrum

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