Effects of fetal calf serum in culture medium on development of bovine oocytes matured and fertilized in vitro.

Effects of fetal calf serum in culture medium on development of bovine oocytes matured and fertilized in vitro. Research Abstract Details 

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Effects of fetal calf serum in culture medium on development of bovine oocytes matured and fertilized in vitro. Abstract Text:

J M Lim,O Okitsu,K Okuda,K Niwa,

Bovine oocytes matured and fertilized in vitro were cultured in a chemically defined bovine embryo culture medium (BECM) or polyvinylalcohol (PVA)-free BECM supplemented with 10% heat-treated or unheated fetal calf serum (FCS). When PVA in BECM was replaced with FCS 8 h postinsemination, the proportion of >/=-cell-stage embryos 48 h postinsemination was significantly lower in heat-treated FCS (74%) than in PVA (91%) medium, but the value (84%) in unheated FCS was not different from the values in PVA and heat-treated FCS. However, the addition of either heat-treated FCS or unheated FCS to PVA-free BECM did not depress or increase the proportions (63 to 70%) of >/=-cell-stage embryos 96 h postinsemination and stimulated blastocyst formation 144 (21 to 28%) and 192 (47 to 54%) h postinsemination. Blastocyst formation was also stimulated when heattreated FCS or unheated FCS was added to PVA-free BECM 120 h postinsemination. The mean cell number of blastocysts developed in PVA-free BECM with heat-treated FCS (150 cells) or unheated FCS (152 cells) significantly increased compared with that (119 cells) developed without FCS. These results indicate that, although BECM can support bovine embryo development, a high proportion (about 50%) of morphologically normal blastocysts can be produced from oocytes matured and fertilized in vitro when they are cultured in PVA-free BECM containing FCS.

Effects of fetal calf serum in culture medium on development of bovine oocytes matured and fertilized in vitro. Publishing Authors By Initials

JM Lim,O Okitsu,K Okuda,K Niwa,

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Effects of fetal calf serum in culture medium on development of bovine oocytes matured and fertilized in vitro. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 41

Page Numbers: 1091-8

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: 05

YEAR: 1994

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LANGUAGE: eng

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Country: United States

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MEDLINETA: Theriogenology

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